Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II

228 kDa

Purified peptide of alpha subunit (amino acids 467-485) of rat type II voltage-gated sodium channel (VGSC) (Accession P04775).

All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

Western blot: 1:200 using ECL on rat brain membranes. Note: The addition of 2% Triton to the antibody is recommended for this lot number.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II detects level of Sodium Channel & has been published & validated for use in IH & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Recognizes type II alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

ImmunoAffinity Purified

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.