biological source
rabbit
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
species reactivity (predicted by homology)
monkey (based on 100% sequence homology), human (based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CSPG4(1464)
mouse ... Cspg4(121021)
rat ... Cspg4(81651)
rhesus monkey ... Cspg4(713086)
General description
NG2是一种高分子量,整合膜硫酸软骨素蛋白聚糖。它在发育中和成熟的中枢神经系统内的一种不常见神经胶质细胞表面上发现,这些细胞具有少突胶质细胞前体细胞(即O-2A祖细胞)的特性。NG2还存在于成软骨细胞,增殖的毛细血管内皮细胞,某些人黑素瘤细胞系以及儿童急性淋巴细胞白血病中的白血病胚细胞表面。NG2蛋白聚糖可能在调节细胞运动,轴突生长和对血小板源性生长因子的细胞反应中起作用。
未偶联的母源抗体(目录号AB5320)观察到分子量为~270-300 kDa
Immunogen
从大鼠中免疫亲和纯化的NG2硫酸软骨素蛋白聚糖
Application
免疫组化分析:代表性批次的1:100的稀释液在成年大鼠脑组织中检测到NG2。
抗NG2,Alexa Fluor™488偶联抗体可检测NG2水平&已发表&经验证可用于ICC &IHC。
研究子类别
神经退行性疾病
神经退行性疾病
研究类别
神经科学
神经科学
Biochem/physiol Actions
该抗体通过WB和ELISA鉴别完整的蛋白聚糖和核心蛋白。当少突胶质细胞前体细胞(即O-2A祖细胞)被活体染色时,该染色会以簇状出现在细胞表面。该抗体不能很好地对分化的少突胶质细胞进行染色。
Physical form
在含0.1%叠氮化钠和15 mg/mLBSA的PBS中,纯化的兔多克隆抗体与Alexa Fluor™488偶联。
蛋白A吸附
Preparation Note
自收到之日起,以未稀释等分试样避光冷藏于 2-8°C,可保存6个月。
Analysis Note
对照
大鼠或人少突胶质细胞前体细胞
大鼠或人少突胶质细胞前体细胞
通过免疫细胞化学在大鼠少突胶质细胞前体细胞中进行评估。
免疫细胞化学分析:该抗体的1:100 的稀释液在大鼠少突胶质细胞前体细胞中检测到NG2。在没有Triton的情况下进行。
免疫细胞化学分析:该抗体的1:100 的稀释液在大鼠少突胶质细胞前体细胞中检测到NG2。在没有Triton的情况下进行。
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Still not finding the right product?
试用我们的 产品选型工具 工具缩小选择范围
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
Anissa Elahi et al.
Cells, 10(11) (2021-11-28)
Following CNS injury, astrocytes become "reactive" and exhibit pro-regenerative or harmful properties. However, the molecular mechanisms that cause astrocytes to adopt either phenotype are not well understood. Transglutaminase 2 (TG2) plays a key role in regulating the response of astrocytes
Michael Munroe et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(6), 7694-7706 (2019-04-26)
Conditions of extended bed rest and limb immobilization can initiate rapid and significant loss of skeletal muscle mass and function. Physical rehabilitation is standard practice following a period of disuse, yet mobility may be severely compromised, and recovery is commonly
Chensu Wang et al.
Cell reports, 37(8), 110021-110021 (2021-11-25)
Treatments aiming to augment immune checkpoint blockade (ICB) in cancer often focus on T cell immunity, but innate immune cells may have important roles to play. Here, we demonstrate a single-dose combination treatment (termed AIP) using a pan-tumor-targeting antibody surrogate, half-life-extended
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| AB5320A4 | 04053252688867 |