biological source
guinea pig
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PDYN(5173)
Immunogen
来自大鼠强啡肽原的合成肽。
Application
免疫组化:1:500
最佳工作稀释度必须由最终用户进行确定。
AB5519应用说明
免疫组织化学
灌注:1)不含Ca2+的台氏液,然后是2)多聚甲醛-苦味酸固定剂和3)10%蔗糖的PBS溶液作为冷冻保护剂。快速解剖组织,并在含有10%蔗糖的0.1 M磷酸盐缓冲液(pH值7.4)中保存过夜。
将载玻片固定的组织切片与封闭缓冲液在室温下培养1小时。将一抗在封闭缓冲液中稀释至适当的工作稀释度。除去封闭缓冲液,然后将载玻片与AB5519在2-8°C下培养18-24小时。在PBS中冲洗3次后,将切片与Cy3偶联的二抗在室温下培养60分钟。固定在含有0.1%对苯二胺的PBS和甘油(1:3)的混合物中后,用Nikon Microphot-SA落射荧光显微镜检查切片。
最佳工作稀释度必须由最终用户进行确定。
AB5519应用说明
免疫组织化学
灌注:1)不含Ca2+的台氏液,然后是2)多聚甲醛-苦味酸固定剂和3)10%蔗糖的PBS溶液作为冷冻保护剂。快速解剖组织,并在含有10%蔗糖的0.1 M磷酸盐缓冲液(pH值7.4)中保存过夜。
将载玻片固定的组织切片与封闭缓冲液在室温下培养1小时。将一抗在封闭缓冲液中稀释至适当的工作稀释度。除去封闭缓冲液,然后将载玻片与AB5519在2-8°C下培养18-24小时。在PBS中冲洗3次后,将切片与Cy3偶联的二抗在室温下培养60分钟。固定在含有0.1%对苯二胺的PBS和甘油(1:3)的混合物中后,用Nikon Microphot-SA落射荧光显微镜检查切片。
抗强啡肽原抗体是针对强啡肽原的抗体,可用于IH。
Biochem/physiol Actions
强啡肽原,大鼠
Physical form
在含 0.05% 叠氮化钠的 PBS 中的亲和纯化豚鼠抗体。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
Prostaglandin E2 acts on EP1 receptor and amplifies both dopamine D1 and D2 receptor signaling in the striatum.
Kitaoka, S; Furuyashiki, T; Nishi, A; Shuto, T; Koyasu, S; Matsuoka, T; Miyasaka et al.
The Journal of Neuroscience null
Repeated exposure to the ?-opioid receptor agonist salvinorin A modulates extracellular signal-regulated kinase and reward sensitivity.
David N Potter,Diane Damez-Werno,William A Carlezon,Bruce M Cohen,Elena H Chartoff
Biological Psychiatry null
The kappa-opioid receptor is primarily postsynaptic: combined immunohistochemical localization of the receptor and endogenous opioids.
Arvidsson, U, et al.
Proceedings of the National Academy of Sciences of the USA, 92, 5062-5066 (1995)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| AB5519 | 04053252621635 |