Anti-P2X7 Receptor Antibody, extracellular domain

Epitope: extracellular domain

Highly purified peptide corresponding to residues 136-152 of mouse P2X7 (Accession Q9Z1M0).

Anti-P2X7 Receptor Antibody, extracellular domain is an antibody against P2X7 Receptor for use in WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blotting: 1:200 using ECL on rat brain membranes.

Flow cytometry, indirect: 5-10 μg antibody per 1x10E6 cells.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Recognizes P2X7, extracellular domain.

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.