Anti-Calpain II Antibody, large subunit

Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF-hand domain IV.
Type I calpain requires micromolar amounts of calcium, while type II calpain requires millimolar amounts of calcium.

Epitope: large subunit

Anti-Calpain II Antibody, large subunit is an antibody against Calpain II for use in WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western blot: 1:1000 for colorimetric substrates or 1:5000 for chemiluminescent substrates (recommended starting concentration).

Optimal working dilutions must be determined by the end user.

The anti-calpain II antibody recognizes the 80 kDa and 58 kDa forms of Calpain II. It does not cross-react with other calpains.

Affinity purified immunoglobulin. Liquid in PBS pH 7.4 containing 1% BSA and 50% glycerol as a stabilizer.

Maintain at -20*C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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