biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Cacng2(12300)
rat ... Cacng2(84347)
Immunogen
来自大鼠细胞质结构域Stargazin的GST融合蛋白。
Application
研究子类别
突触&突触生物学
突触&突触生物学
研究类别
神经科学
神经科学
蛋白质印迹:0.1 μg/mL,大鼠脑的Triton可溶性部分。建议的稀释缓冲液是TBS-T,封闭缓冲液是3%脱脂牛奶。建议培养时间为室温下3小时过夜。
免疫组化:0.25-0.5 μg/mL,4%多聚甲醛固定组织。建议的稀释缓冲液是PBS-T。建议的透化方法是0.1% Triton X-100。建议培养时间为室温下2小时过夜。
免疫细胞化学
免疫沉淀:1-2 μg。建议反应容积为200-1000 μL。建议总蛋白浓度为2 mg/mL。建议培养时间为2-8°C下2小时。建议捕捉剂为蛋白A。
最佳工作稀释度必须由最终用户进行确定。
免疫组化:0.25-0.5 μg/mL,4%多聚甲醛固定组织。建议的稀释缓冲液是PBS-T。建议的透化方法是0.1% Triton X-100。建议培养时间为室温下2小时过夜。
免疫细胞化学
免疫沉淀:1-2 μg。建议反应容积为200-1000 μL。建议总蛋白浓度为2 mg/mL。建议培养时间为2-8°C下2小时。建议捕捉剂为蛋白A。
最佳工作稀释度必须由最终用户进行确定。
该抗Stargazin抗体经验证可用于IP、WB、IC、IH中Stargazin的检测。
Biochem/physiol Actions
Stargazin [Stargazin样TARP(跨膜AMPA受体调节蛋白)]。 通过蛋白质印迹法,该抗体可识别大鼠脑Triton可溶性部分~ 35kda和~ 55kda的条带。该抗体主要与TARP γ -8反应,与γ -3和γ -4反应弱。Stargazin在海马体和小脑中强烈表达。
Physical form
免疫亲和纯化
形式:纯化
液体形式,含130 mM Tris-HCl、87 mM甘氨酸,pH 7.4。
Preparation Note
接收后,以未稀释的等分试样在-80°C下保存长达6个月。应避免反复冻融循环。解冻后抗体可在2-8°C下保存1-3周。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Susumu Tomita et al.
The Journal of cell biology, 161(4), 805-816 (2003-05-29)
Functional expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in cerebellar granule cells requires stargazin, a member of a large family of four-pass transmembrane proteins. Here, we define a family of transmembrane AMPA receptor regulatory proteins (TARPs), which comprise stargazin, gamma-3, gamma-4
Susumu Tomita et al.
Science (New York, N.Y.), 303(5663), 1508-1511 (2004-03-06)
Activity-dependent plasticity in the brain arises in part from changes in the number of synaptic AMPA receptors. Synaptic trafficking of AMPA receptors is controlled by stargazin and homologous transmembrane AMPA receptor regulatory proteins (TARPs). We found that TARPs were stable
Susumu Tomita et al.
Neuron, 45(2), 269-277 (2005-01-25)
Synaptic plasticity involves protein phosphorylation cascades that alter the density of AMPA-type glutamate receptors at excitatory synapses; however, the crucial phosphorylated substrates remain uncertain. Here, we show that the AMPA receptor-associated protein stargazin is quantitatively phosphorylated and that stargazin phosphorylation
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| AB9876 | 04053252408595 |