biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
ChIP: suitable (ChIP-seq), immunofluorescence: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Lhx2(16870)
General description
Immunogen
Application
Western Blotting Analysis: A 1:4,000 dilution from a representative lot detected LHX2 in lysate from doxycycline treated murine K14rtTA-LHX2-TRE keratinocytes and human brain tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected LHX2 in mouse embryo tissue sections.
Immunofluorescence Analysis: A representative lot of this antibody detected LHX2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University; Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
ChIP seq Analysis; A representative lot of this antibody detected LHX2 by ChIP-seq analysis (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
染色质免疫沉淀分析:一个有代表性的批次在来自处于第二个休止期的小鼠的富含毛囊干细胞(HF-SCs)的 α6hi/CD34+ 皮肤细胞中检测到 Lhx2 靶向的启动子和增强子区域(Folgueras, A.R., et. al.(2013).Cell Stem Cell.13(3):314-327)。
染色质免疫沉淀测序分析:一个有代表性批次通过全基因组 ChIP-seq 分析在处于第二个休止期的小鼠的富含毛囊干细胞(HF-SCs)的 α6hi/CD34+ 皮肤细胞中检测到了 Lhx2 靶向的启动子和增强子区域(Folgueras, A.R., et. al.(2013).Cell Stem Cell.13(3):314-327)。
免疫荧光分析:一个有代表性批次以 1:1,000 的稀释度通过荧光免疫组化在 OCT 包埋的、PFA 固定的人皮肤样品的冷冻切片中检测到了 Lhx2 免疫反应性(数据由洛克菲勒大学霍华德·休斯医学研究所的 Elaine Fuchs 博士提供)。
核受体
表观遗传学&核功能
Physical form
Preparation Note
处理建议:收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。避免反复冻融循环,以免损坏 IgG 和影响产品性能。
Analysis Note
蛋白质印迹分析: 该抗体以 1:5,000 的稀释度在 10 µg 小鼠角质形成细胞 K14rtTA-LHX2-TRE 细胞裂解液中检测到了强力霉素诱导的 LHX2 表达。
Other Notes
Disclaimer
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
相关内容
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| ABE1402 | 04055977287912 |