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Merck
CN

ABE1402

抗LHX2抗体

serum, from rabbit

别名:

LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein, LHX2

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ChIP, IF, WB
Citations:
5
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

technique(s)

ChIP: suitable (ChIP-seq), immunofluorescence: suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Lhx2(16870)

General description

LIM/同源盒蛋白 Lhx2(UniProt Q9Z0S2;也称为同源盒蛋白 LH-2、LIM 同源盒蛋白 2、Lim2)在鼠类中由 Lhx2 基因(基因 ID 16870)编码。Lhx2 是一种转录因子,在控制皮肤毛发循环、眼睛、前脑、肢体、造血、嗅觉和垂体系统发育的祖细胞的行为中起重要作用。经证明,Lhx2 可调节大量毛囊干细胞(HF-SC)签名基因,以及龛位内的细胞骨架和粘附分子,在龛位中 HF-SCs 是单层极化的静态上皮细胞。Lhx2 缺乏症会导致脱发,因为由于 HF-SC 的极化和龛位结构受到严重干扰,龛位不再能够锚定其头发并维持其 SC 的适当行为。
观察到的分子量:~48 kDa由于翻译后糖基化修饰,目标条带大小似乎大于计算的 44.4 kDa 分子量。某些裂解液可能会出现未表征的条带。

Immunogen

对应于小鼠 LHX2 的重组蛋白。

Application

Tested Applications

Western Blotting Analysis: A 1:4,000 dilution from a representative lot detected LHX2 in lysate from doxycycline treated murine K14rtTA-LHX2-TRE keratinocytes and human brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected LHX2 in mouse embryo tissue sections.

Immunofluorescence Analysis: A representative lot of this antibody detected LHX2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University; Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).

ChIP seq Analysis; A representative lot of this antibody detected LHX2 by ChIP-seq analysis (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
免疫荧光分析:一个有代表性的批次通过荧光免疫组化在 OCT 包埋的、PFA 固定的野生型小鼠(来自不同发育阶段,包括 E16.5、P0、P30 和第二个休止期)背部皮肤冷冻切片中检测到 Lhx2 免疫反应性,但在 Lhx2 基因敲除的小鼠切片中检测不到。(Folgueras, A.R., et. al.(2013).Cell Stem Cell.13(3):314-327)。
染色质免疫沉淀分析:一个有代表性的批次在来自处于第二个休止期的小鼠的富含毛囊干细胞(HF-SCs)的 α6hi/CD34+ 皮肤细胞中检测到 Lhx2 靶向的启动子和增强子区域(Folgueras, A.R., et. al.(2013).Cell Stem Cell.13(3):314-327)。
染色质免疫沉淀测序分析:一个有代表性批次通过全基因组 ChIP-seq 分析在处于第二个休止期的小鼠的富含毛囊干细胞(HF-SCs)的 α6hi/CD34+ 皮肤细胞中检测到了 Lhx2 靶向的启动子和增强子区域(Folgueras, A.R., et. al.(2013).Cell Stem Cell.13(3):314-327)。
免疫荧光分析:一个有代表性批次以 1:1,000 的稀释度通过荧光免疫组化在 OCT 包埋的、PFA 固定的人皮肤样品的冷冻切片中检测到了 Lhx2 免疫反应性(数据由洛克菲勒大学霍华德·休斯医学研究所的 Elaine Fuchs 博士提供)。
研究子类别
核受体
研究类别
表观遗传学&核功能
该抗 LHX2 抗体经过验证可用在蛋白印迹、免疫荧光、ChIP-seq 和染色质免疫沉淀(ChIP)中检测 LHX2。

Physical form

含 0.05% 叠氮化钠的兔多克隆血清。
未纯化

Preparation Note

自收到之日起,在 -20°C 条件下可稳定保存 1 年。
处理建议:收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。避免反复冻融循环,以免损坏 IgG 和影响产品性能。

Analysis Note

通过蛋白质印迹在鼠角质形成细胞 K14rtTA-LHX2-TRE 细胞裂解液中进行了评估。

蛋白质印迹分析: 该抗体以 1:5,000 的稀释度在 10 µg 小鼠角质形成细胞 K14rtTA-LHX2-TRE 细胞裂解液中检测到了强力霉素诱导的 LHX2 表达。

Other Notes

浓度:请参考特定批次的数据表。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Alicia R Folgueras et al.
Cell stem cell, 13(3), 314-327 (2013-09-10)
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全球贸易项目编号

货号GTIN
ABE140204055977287912