biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer300)
Gene Information
human ... PDHA1(5160)
General description
在许多生物体中,丙酮酸脱氢酶复合体除了作为心脏底物选择的关键调节因子外,还在有氧能量生成途径中催化丙酮酸到乙酰辅酶a和二氧化碳的总体不可逆转化。它含有三种酶成分的多个副本:丙酮酸脱氢酶(E1)、二氢脂酰胺乙酰转移酶(E2)和脂酰胺脱氢酶(E3)。PDH通过丙酮酸脱氢酶激酶(PDK)介导的磷酸化和反馈抑制调节。
观测值〜43 kDa
Immunogen
对应于在Ser300磷酸化的1型人PDHE1-A的KLH偶联线性肽。
表位:磷酸化Ser300
Application
免疫细胞化学分析: 代表性批次的1:500稀释液在二氯乙酸未处理和已处理的HEK 293细胞中检测到PDHE1-A 1型。
该抗磷酸化PDHE1-A 1型(Ser300)抗体经验证可用于在WB、IC中检测磷酸化PDHE1-A 1型(Ser300)。
Biochem/physiol Actions
该抗体可识别在Ser300磷酸化的PDHE1-A 1型。
Analysis Note
对照
二氯乙酸未处理和已处理的HEK 293细胞裂解液
二氯乙酸未处理和已处理的HEK 293细胞裂解液
已通过蛋白质印迹在二氯乙酸未处理和已处理的NIH/3T3细胞裂解液中进行了评估。
蛋白质印迹分析:0.5 µg/mL该抗体在10 µg二氯乙酸未处理和已处理的HEK 293细胞裂解液中检测到PDHE1-A 1型。
蛋白质印迹分析:0.5 µg/mL该抗体在10 µg二氯乙酸未处理和已处理的HEK 293细胞裂解液中检测到PDHE1-A 1型。
Other Notes
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Hao Shi et al.
Molecular metabolism, 11, 160-177 (2018-03-12)
Given that cellular O-GlcNAcylation levels are thought to be real-time measures of cellular nutrient status and dysregulated O-GlcNAc signaling is associated with insulin resistance, we evaluated the role of O-GlcNAc transferase (OGT), the enzyme that mediates O-GlcNAcylation, in skeletal muscle.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| ABS194 | 04053252633966 |