Anti-DEPTOR Antibody

DEPTOR (DEPDC6, DEP domain-containing protein 6) was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR (Peterson, T.R., et al. (2009). Cell. 137(5):873-876).

~46 kDa observed.

Epitope: DEP domain-containing mTOR-interacting protein DEPTOR.

KLH-conjugated linear peptide corresponding to the DEP domain-containing mTOR-interacting protein of human DEPTOR.

Anti-DEPTOR Antibody detects level of DEPTOR & has been published & validated for use in WB.

Research Category
Metabolism

Research Sub Category
PI3K, Akt, & mTOR Signaling

Other homologies: Primate, Equine, Porcine, Canine, Rat, and Mouse based on 99%, 99%, 98%, 97%, and 96% homology, respectively.

This antibody recognizes the DEP domain that contains mTOR-interacting protein DEPTOR.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK293 cell lyaste

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DEPTOR in HEK293 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 09-463

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