biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
rat ... Cyp11A1(29680)
General description
As a member of the cytochrome P450 family, Cytochrome P450 Side Chain Cleavage Enzyme (scc) catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone during the first step of steroid biosynthesis. It is expressed in certain organs, such as ovaries, adrenal glands, and testis. Deficiency in CYP11A1 is commonly associated with adrenal insufficiency, and with variable degrees of disorder of sex development (DSD).
~52 kDa observed. On higher percentage gels and longer exposures, 8 kDa cleavage product can be detected.
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of rat Cytochrome P450 (scc), a.a. 509-526.
Application
Immunohistochemistry Analysis: A 1:4,000-8,000 dilution from a representative lot detected Cytochrome P450 (scc) in rat adrenal gland and rat testis tissue.
This Cytochrome P450 (scc) antibody is validated for use in WB & IHC for the detection of the Cytochrome P450 (scc) protein.
Biochem/physiol Actions
This antibody recognizes the C-terminus of Cytochrome P450 (scc), a.a. 509-526.
Analysis Note
Control
Rat adrenal tissue lysate
Rat adrenal tissue lysate
Evaluated by Western Blot in rat adrenal tissue lysate.
Western Blot Analysis: 0.02 µg/mL of this antibody detected Cytochrome P450 (scc) in 10 µg of rat adrenal tissue lysate.
Western Blot Analysis: 0.02 µg/mL of this antibody detected Cytochrome P450 (scc) in 10 µg of rat adrenal tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB1294
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Li Guang-Yu et al.
Molecular reproduction and development, 83(3), 226-235 (2016-03-21)
Myeloid cell leukemia-1 (MCL1), an anti-apoptotic member of the BCL2 family, is expressed abundantly in the testis. Previous characterization revealed that MCL1 is expressed exclusively in the Leydig cells in the mouse testis, yet what it does in these cells
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| ABS236 | 04053252847738 |