Anti-phospho NFkB p65 Antibody, (Ser547)

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa B forms multiple homo and heterodimeric complexes, one of which is with RELA/p65. The NF-kappa-B p65 complex appears to be involved in invasin-mediated activation of IL-8 expression, as well as the inhibitor effect on I-kappa-B. Phosphorylation of p65 at serine 547 by the nuclear kinase ATM appears to repress NF-κB-dependent transcription of specific genes induced by genotoxic stress.

~62 kDa observed. Uncharacterized band(s) may appear in some lysates.

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human phospho NFkB p65, (Ser547).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Anti-phospho NFkB p65 Antibody, (Ser547) is an antibody against phospho NFkB p65 Antibody for use in Western Blotting.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in p65 WT transfected U2OS cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho NFkB p65, (Ser547) in 10 µg of p65 WT transfected U2OS cell lysate.

Concentration: Please refer to lot specific datasheet.

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