biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized
species reactivity
human
manufacturer/tradename
Chemicon®
concentration
1.0 mg/mL
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
免疫球蛋白G(IgG)是人血清中发现的主要的糖蛋白抗体类型,由B细胞分泌。其在血液中发现,包含四条多肽链,由两条相同的50 kDaγ重(H)链和两条相同的25 kDaκ或λ轻(L)链组成。这些链通过二硫键相互连接。IgG以IgG1、IgG2、IgG3和IgG4四种亚型存在。与其他免疫球蛋白相比,IgG的血清半衰期最长。
Application
ELISA: 1:3,000
免疫组化:1:200-1:500(1-4)
免疫细胞化学: 1:200-1:500(1-4)
蛋白质印迹,显色: 1:200-1:500
蛋白质印迹,化学发光: 1:2,000-1:5,000
最佳工作稀释度必须由最终用户确定。
免疫组化:1:200-1:500(1-4)
免疫细胞化学: 1:200-1:500(1-4)
蛋白质印迹,显色: 1:200-1:500
蛋白质印迹,化学发光: 1:2,000-1:5,000
最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
免疫球蛋白G(IgG)启动补体系统的经典途径。它中和病毒颗粒和毒素。IgG在抗体依赖性细胞介导的细胞毒性(ADCC)中起着至关重要的作用,并且可能与过敏有关。母体IgG通过胎盘传递给胎儿,对于新生儿免疫防御感染至关重要。木瓜蛋白酶消化IgG导致产生片段抗原结合(Fab)。IgG的胃蛋白酶消化导致可结晶片段(Fc)的产生。严重急性呼吸综合征冠状病毒(SARS-CoV)-2感染患者的IgG水平可作为评估2019冠状病毒(COVID-19)严重程度和预后的潜在工具。
对人IgG γ链具有特异性。 采用ELISA法检验抗人IgG(γ)抗体的交叉反应性。 与轻链的交叉反应性极低。
该试剂是来源于山羊的亲和纯化抗体。 纯化的抗体与辣根过氧化物酶(HRP)偶联,然后在缓冲液中稳定化。
该试剂是来源于山羊的亲和纯化抗体。 纯化的抗体与辣根过氧化物酶(HRP)偶联,然后在缓冲液中稳定化。
Physical form
免疫亲和纯化
0.01 M PBS,pH7.1,含15mg/ml BSA
0.01 M PBS,pH7.1,含15mg/ml BSA
Preparation Note
未稀释的抗体在2-8°C下可稳定保存12个月。请勿稀释后保存。不可冷冻。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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