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Merck
CN

CBL154

Anti-H-CAM Antibody, clone F10-44-2

clone F10-44-2, Chemicon®, from mouse

别名:

CD44

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
F10-44-2, monoclonal
Application:
FACS, IHC (p), IP
Citations:
7
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

F10-44-2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable

isotype

IgG2a

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD44(960)

Application

Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).

Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.

Studies on HCAM (homing receptor) function.

Immunoprecipitation.

Optimal working dilutions must be determined by the end user.
This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM.

Physical form

Format: Purified

Analysis Note

Control
POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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实验方案

有关间充质的信息,特别是间充质干细胞培养实验方案用于间充质干细胞(MSC)分离、扩增和分化的分步细胞培养方案。

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

商品

新型无血清、无异种人间充质干细胞(MSC)骨细胞分化培养基的开发。

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.


Biochemical characterisation and localization in brain of a human brain-leucocyte membrane glycoprotein recognised by a monoclonal antibody.
McKenzie, J L, et al.
Journal of Neurochemistry, 39, 1461-1466 (1982)
Identification of mRNA that encodes an alternative form of H-CAM(CD44) in lymphoid and nonlymphoid tissues.
Goldstein, L A and Butcher, E C
Immunogenetics, 32, 389-397 (1990)
José M Díez et al.
Stem cell research & therapy, 6, 28-28 (2015-04-19)
Fetal bovine serum (FBS) is an animal product used as a medium supplement. The animal origin of FBS is a concern if cultured stem cells are to be utilized for human cell therapy. Therefore, a substitute for FBS is desirable.



全球贸易项目编号

货号GTIN
CBL15404053252583971