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Merck
CN

CBL218

Anti-Keratin K3/K76 抗体,克隆 AE5

clone AE5, Chemicon®, from mouse

别名:

Anti-HUMCYT2A, Anti-KRT2P

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
AE5, monoclonal
Application:
IHC (p), WB
Citations:
21
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

AE5, monoclonal

species reactivity

human, mouse, bovine, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KRT3(3850)

General description

64 kDa

Immunogen

兔角膜上皮角蛋白

Application

使用冷冻切片进行的免疫组化:1:50。

使用石蜡包埋切片进行的免疫组化:在胰蛋白酶消化抗原修复后,将石蜡包埋的组织切片按1:50比例固定在10%中性缓冲福尔马林或Fekete’s酸-酒精-福尔马林中(Mikaelian et al.,2004)。

蛋白质印迹:1:500。

最佳工作稀释度必须由最终用户进行确定。
抗角蛋白K3/K76抗体,克隆AE5是抗角蛋白K3/K76的抗体,用于WB,IH,IH(P)。

Biochem/physiol Actions

该抗体与角膜上皮的64 kDa多肽(细胞角蛋白3;角蛋白K3)和上颚上皮的角蛋白K76(细胞角蛋白K2p)反应。 因此,这代表了研究角膜上皮类型分化的极佳标记。也可识别牛口鼻和唇上皮以及兔角膜细胞系。

Physical form

形式:纯化
通过离子交换色谱法纯化单克隆抗体,将其置于含有0.09%叠氮化钠的磷酸盐缓冲生理盐水(pH 7.2)中。

Preparation Note

若购买后1个月内使用,则储存于2°-8°C,若要长期储存,则将抗体等分成小体积并储存于-20°C,保存达1年。

Other Notes

浓度:请参考批次特异性浓缩物的检验报告。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

存储类别

12 - Non Combustible Liquids



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A biomimetic scaffold for culturing limbal stem cells: a promising alternative for clinical transplantation.
Subhadra Dravida, Subhash Gaddipati, May Griffith, Kim Merrett, Soundarya Lakshmi Madhira et al.
Journal of Tissue Engineering and Regenerative Medicine null
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PloS one, 8(3), e60016-e60016 (2013-03-26)
Human pluripotent stem cells (hPSCs) provide unprecedented opportunities to study the earliest stages of human development in vitro and have the potential to provide unlimited new sources of cells for regenerative medicine. Although previous studies have reported cytokeratin 14+/p63+ keratinocyte
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Schermer, A; Galvin, S; Sun, TT
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全球贸易项目编号

货号GTIN
CBL21804053252361777