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Merck
CN

CBL582

Anti-CD19 Antibody, clone HD37

clone HD37, Chemicon®, from mouse

别名:

B-lymphocyte Surface Antigen B4

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
HD37, monoclonal
Application:
FACS, IHC, IP
Citations:
4
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

HD37, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD19(930)

Application

Anti-CD19 Antibody, clone HD37 detects level of CD19 & has been published & validated for use in FC, IP, IH.
Identification of CD19 positive B lymphocytes and malignant B cells by indirect immunofluorescence staining

Leukaemia and lymphoma phenotyping

Identification of normal and malignant CD19 positive B lymphocytes in cryostat frozen sections

Immunoprecipitation of the CD19 antigen

Studies of the CD19/21 complex in B cell activation and the control of B cell development

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

The CD19 antigen (90 kDa) is expressed from the earliest stages of B-progenitor development, on all peripheral B cells including germinal centre B cells and all B cell lines and B cell leukaemias tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturations to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.

Physical form

Format: Purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Other Notes

Replaces: CBL1322

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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G F Fischer et al.
Journal of immunology (Baltimore, Md. : 1950), 144(2), 638-641 (1990-01-15)
The CD24 Ag is present on human B cells from the earliest stages of B lineage development and is lost on terminal B cell maturation to plasma cells. This Ag is also expressed on mature forms of granulocytes. We studied
Gladys S Ingle et al.
British journal of haematology, 140(1), 46-58 (2007-11-10)
CD19 and CD21 (CR2) are co-receptors found on B-cells and various B-cell lymphomas, including non-Hodgkin lymphoma. To evaluate their suitability as targets for therapy of such lymphomas using internalization-dependent antibody-drug conjugates [such as antibody-4-(N-maleimidomethyl)cyclohexane-1-carboxylate, (N2'-deacetyl-N2'-(3-mercapto-1-oxopropyl)-maytansine) (MCC-DM1) conjugates, which require lysosomal
Uwe Reusch et al.
mAbs, 7(3), 584-604 (2015-04-16)
To harness the potent tumor-killing capacity of T cells for the treatment of CD19(+) malignancies, we constructed AFM11, a humanized tetravalent bispecific CD19/CD3 tandem diabody (TandAb) consisting solely of Fv domains. The molecule exhibits good manufacturability and stability properties. AFM11



全球贸易项目编号

货号GTIN
CBL58204053252512643