biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
DH01, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (1 µg/mL)
Immunoprecipitation (2 µg/mg protein lysate))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CDH1(999)
General description
抗CDH1（Ab-2），小鼠单克隆，克隆DH01，可识别大鼠1细胞中约55 kDa Cdh-1蛋白。它已被验证可用于蛋白质印迹和免疫沉淀。
用指定的免疫原免疫BALB/c小鼠，并将脾细胞与Sp2/0小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体。识别约为55 kDa的ID1蛋白。
识别大鼠1细胞中~55 kDa Cdh-1蛋白。
Immunogen
人
重组人Cdh1蛋白
Application
免疫印迹（1 g/mL）
免疫沉淀（2 g/mg蛋白裂解物）
Packaging
请参考特定浓度批号的标签。
Physical form
在10 mM PBS，0.2%BSA，pH值7.4中。
Analysis Note
阳性对照
：KAT或LS174T细胞
：KAT或LS174T细胞
阴性对照
正常小鼠血清
正常小鼠血清
Other Notes
Kramer, E.R., et al. 2000 Mol.Biol. Cell11, 1555.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol.Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc.Natl.Acad.Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol.Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc.Natl.Acad.Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.
不与其他含有WD重复序列的蛋白质（包括Cdc20）发生交叉反应。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
Still not finding the right product?
试用我们的 产品选型工具 工具缩小选择范围
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Dipankar Ray et al.
Molecular and cellular biology, 25(8), 3338-3347 (2005-03-31)
Ubiquitin-dependent degradation of Cdc25A is a major mechanism for damage-induced S-phase checkpoint. Two ubiquitin ligases, the Skp1-cullin-beta-TrCP (SCFbeta-TrCP) complex and the anaphase-promoting complex (APCCdh1), are involved in Cdc25A degradation. Here we demonstrate that the transforming growth factor beta (TGF-beta)-Smad3 pathway
Jia Liu et al.
Cell discovery, 2, 15044-15044 (2016-07-28)
Anaphase-promoting complex/cyclosome/Cdh1 is a multi-subunit ubiquitin E3 ligase that drives M to G1 cell cycle progression through primarily earmarking various substrates for ubiquitination and subsequent degradation by the 26S proteasome. Notably, emerging evidence suggested that Cdh1 could also function in
Kyung Uk Hong et al.
The Journal of biological chemistry, 284(24), 16501-16512 (2009-04-17)
During mitosis, establishment of structurally and functionally sound bipolar spindles is necessary for maintaining the fidelity of chromosome segregation. Tumor-associated microtubule-associated protein (TMAP), also known as cytoskeleton-associated protein 2 (CKAP2), is a mitotic spindle-associated protein whose level is frequently up-regulated
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CC43-100UG | 04055977228472 |