biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
II-4C11, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human, bovine, rabbit
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1-10 µg/mL)
Immunoblotting (1-5 µg/mL,
Paraffin Sections (1-10 µg/mL, )
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... COL2A1(1280)
General description
可识别约130 kDa的II型胶原蛋白。可能与其他胶原发生反应。当以高浓度使用时，也可以检测到对应于聚集体的更高条带。
经验证，该抗II型胶原（Ab-1）小鼠mAb（II-4C11）可用于冰冻切片检测II型胶原（Ab-1）。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与P3-X-63-Ag8-LUI小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体。可识别约130 kDa的II型胶原蛋白。
Immunogen
人
纯化的II型胶原
Application
冷冻切片（1-10 µg/ml）
免疫印迹（1-5 µg/ml，请参阅应用参考）
石蜡切片（1-10 µg/ml，请参阅应用参考）
免疫印迹（1-5 µg/ml，请参阅应用参考）
石蜡切片（1-10 µg/ml，请参阅应用参考）
Packaging
请参考特定浓度批号的标签。
Physical form
溶于100 mM磷酸钠缓冲液，0.1% BSA（pH 7.0）。
Preparation Note
初次融化后，等分并冷冻（-20°C）。
Other Notes
Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I.1993 Ann.Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell.Biol.114, 1307.
Ryan, M.C., and Sandell, L.J.1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E.1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
Kivrikko, K,I.1993 Ann.Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell.Biol.114, 1307.
Ryan, M.C., and Sandell, L.J.1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E.1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
可能与其他胶原发生反应。当以高浓度使用时，也可以检测到对应于聚集体的更高条带。该抗体也可以在高于1 µg/mL的浓度下与其他类型的胶原反应。在免疫印迹中，获得了约130 kDa的主要条带。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
由Daiichi Fine Chemical Co.，Ltd.生产，未在日本销售。
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CP18-100UG | 04055977220957 |