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EZHS40

Human Amyloid β40 ELISA Kit

measures and quantifies Amyloid β40 levels in 50 μL CSF, cell culture supernatent or plasma

别名:

Amyloid-beta protein 40

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关于此项目

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Gene information:
human ... APP(351)
Input:
sample type plasma (K2 EDTA)
sample type serum
sample type cerebrospinal fluid (CSF)
Assay range:
sensitivity: 6.0 pg/mL
(50 μl sample size), standard curve range: 16-500 pg/mL
Species reactivity:
human
Storage temp.:
2-8°C
Shipped in:
wet ice
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产品名称

High Sensitivity Human Amyloid β40 ELISA,

Quality Level

description

This High Sensitivity Human Amyloid β40 ELISA is used to measure & quantify Amyloid β40 levels in Neuroscience research.

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

50 μL sample volume (Overnight assay)

assay range

sensitivity: 6.0 pg/mL
(50 μl sample size), standard curve range: 16-500 pg/mL

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum
sample type cerebrospinal fluid (CSF)

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... APP(351)

General description

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s High Sensitivity Human Amyloid β40 ELISA kit is used for the measurement of Amyloid β40 in cerebrospinal fluid, cell culture supernatants, primary neurons and plasma in a 96-well format.

Application

Research Category
Neuroscience
Research Sub Category
Alzheimer′s Disease
This High Sensitivity Human Amyloid β40 ELISA is used to measure & quantify Amyloid β40 levels in Neuroscience research.
This assay requires 50 µl of sample (cerebrospinal fluid, cell culture supernatants, primary neurons, or plasma) and is an overnight assay.
Used to detect/quantify: Amyloid β40

Biochem/physiol Actions

The Amyloid β40 ELISA (HS) uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

Preparation Note

Components in the kit can be stored up to 2 weeks at 2-8°C

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.


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Warning

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

wgk

WGK 3

存储类别

10 - Combustible liquids



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Kendra L Puig et al.
Journal of Alzheimer's disease : JAD, 44(4), 1263-1278 (2014-11-20)
Alzheimer's disease (AD) is a neurodegenerative disorder histologically characterized by amyloid-β (Aβ) protein accumulation and activation of associated microglia. Although these features are well described in the central nervous system, the process and consequences of Aβ accumulation in the enteric
Loukia Katsouri et al.
Neurobiology of aging, 34(4), 1105-1115 (2012-10-16)
Noradrenergic deficits have been described in the hippocampus and the frontal cortex of Alzheimer's disease brains, which are secondary to locus coeruleus degeneration. Locus coeruleus is the brain stem nucleus responsible for synthesis of noradrenaline and from where all noradrenergic
Mariya V Hrynchak et al.
Frontiers in synaptic neuroscience, 12, 16-16 (2020-05-12)
Alzheimer's disease is regarded as a synaptopathy with a long presymptomatic phase. Soluble, oligomeric amyloid-β (Aβ) is thought to play a causative role in this disease, which eventually leads to cognitive decline. However, most animal studies have employed mice expressing



全球贸易项目编号

货号GTIN
EZHS4004053252328282