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GR01

Anti-EGFR (Ab-1) Mouse mAb (528)

liquid, clone 528, Calbiochem®

别名:

Anti-Epidermal Growth Factor Receptor

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关于此项目

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
528, monoclonal
Species reactivity:
human
Application:
—
Citations:
4
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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

528, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

should not react with

mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Flow Cytometry (5 µg/mL)
Frozen Sections (2.5 µg/mL)
Immunofluorescence (5 µg/mL)
Immunoprecipitation(1 µg/sample)
Neutralization Studies
Paraffin Sections )

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)

General description

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.
Recognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation(1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Negative Control
HL-60 cells or human brain
Positive Control
A-431 or normal skin tissue

Other Notes

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Jareer Kassis et al.
International journal of cancer, 99(5), 644-651 (2002-07-13)
Tumor invasion marks a critical point in cancer progression; it is a harbinger of morbidity and mortality. Thus, the cellular events that enable the invasive phenotype are under intense investigation. Epstein-Barr virus (EBV) is associated with a number of cancers
Valencio Salema et al.
mAbs, 8(7), 1286-1301 (2016-07-30)
Most therapeutic antibodies (Abs) target cell surface proteins on tumor and immune cells. Cloning of Ab gene libraries in E. coli and their display on bacteriophages is commonly used to select novel therapeutic Abs binding target antigens, either purified or
Inmaculada Bañón-Rodríguez et al.
The EMBO journal, 33(2), 129-145 (2014-01-15)
Establishing the correct orientation of the mitotic spindle is an essential step in epithelial cell division in order to ensure that epithelial tubules form correctly during organ development and regeneration. While recent findings have identified some of the molecular mechanisms
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全球贸易项目编号

货号GTIN
GR01-100UG04055977220162