GR37
Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)
liquid, clone 9A7γE10.4, Calbiochem®
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
9A7γE10.4, monoclonal
Species reactivity:
chicken, hamster, mouse, porcine, rat, amphibian, human
Application:
—
Citations:
1
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
9A7γE10.4, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
chicken, hamster, mouse, porcine, rat, amphibian, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1:50-1:100)
Gel Shift
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µL/mg protein lysate)
Paraffin Sections (1:50-1:100, sample pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... VDR(7421)
General description
Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.
Recognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.
This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).
Immunogen
purified, chick intestine vitamin D receptor
Application
Frozen Sections (1:50-1:100)
Gel Shift (see comments)
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Gel Shift (see comments)
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
Breast carcinoma tissue (see comments)
Breast carcinoma tissue (see comments)
Other Notes
Brown, A.J. 1999. Nephrol. Dial. Transplant.14, 11.
Fleet, J.C. 1999. Nutr. Rev.57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res.47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.
Fleet, J.C. 1999. Nutr. Rev.57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res.47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.
Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
José L Costa et al.
BMC genomics, 10, 499-499 (2009-10-30)
The active form of Vitamin D, 1,25-dihydroxyvitamin D3 (1,25D), has strong anti-proliferative effects, yet the molecular mechanisms underneath this effect remain unclear. In contrast, the molecular mechanism of 1,25D for the regulation of calcium homeostasis has principally been resolved, demonstrating
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GR37-100UG | 04055977228311 |