biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
42-5D11, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
bovine, rat, mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections
Immunoblotting (1 µg/mL)
Paraffin Sections )
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MMP2(4313)
General description
Purified mouse monoclonal antibody (see application references). Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
Recognizes the ~72 kDa latent and the ~66 kDa active forms of the MMP-2.
This Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-2 (Ab-3).
Immunogen
Epitope: within amino acids 468-483 of human MMP-2 (numbered from the propeptide)
Human
a synthetic peptide (VTPRDKPMGPLLVATF) corresponding to amino acids 468-483 of human MMP-2
Application

Frozen Sections (see application references)
Immunoblotting (1 g/ml)
Paraffin Sections (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
MMP-9 protein (Cat. Nos. PF024 or PF038)
MMP-9 protein (Cat. Nos. PF024 or PF038)
Positive Control
MMP-2 protein (Cat. Nos. PF023 or PF037)
MMP-2 protein (Cat. Nos. PF023 or PF037)
Other Notes
Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol.2, 861.
Fujimoto, N., et al. 1993. Clinica Chimica Acta.221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Woessner, J. F. 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.
Fujimoto, N., et al. 1993. Clinica Chimica Acta.221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Woessner, J. F. 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.
This antibody will not cross-react with human MMP-1, MMP-3, MMP-9, or MMP-13. Antibody should be titrated for optimal results in individual systems.
Legal Information
Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Peter McCluskey et al.
Investigative ophthalmology & visual science, 50(5), 2161-2164 (2009-01-02)
To determine whether MMPs and TIMPs are present in the bleb wall of Molteno implants. An observational case series consisting of ocular specimens from 10 human eyes obtained postmortem from patients who had undergone placement of Molteno implants for glaucoma.
null
Laura S Spruill et al.
American journal of physiology. Cell physiology, 293(4), C1362-C1373 (2007-08-03)
Past studies have identified that a unique type of matrix metalloproteinase, the membrane-type-1 MMP (MT1-MMP), is increased within the left ventricle (LV) of patients with dilated cardiomyopathy (DCM). However, the cellular and molecular basis for this induction of MT1-MMP with
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| IM33-100UG | 07790788053772 |