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IPVH00010

Immobilon® -P PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane

别名:

Western blotting membrane, blotting membrane, transfer membrane

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关于此项目

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
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产品名称

Immobilon®-P PVDF膜, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Level

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

General description

Immobilon-P转印膜推荐用于大多数蛋白质印迹应用，尤其是大于20 kDa的蛋白质。Immobilon-P膜具有出色的蛋白质保留率、高物理强度和广泛的化学相容性，因而成为各种染色应用及免疫检测方法再生检测的理想选择。

Features and Benefits

Immobilon-P PVDF膜：
  • 切割时不会开裂、卷曲或断裂
  • 低背景
  • 优异的染色能力
  • 可多次再生检测

Other Notes

主要结合机制：静电，疏水
替代品：INCP00010
滤器编码：IPVH

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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实验方案

免疫印迹膜剥离和再生检测

本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

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全球贸易项目编号

货号GTIN
IPVH0001008436037122422
WBPV0100404053252026515
WBPV0102004053252026522