跳转至内容
Merck
CN

MAB10753

抗半胱天冬酶3(活性形式)抗体,克隆3D9.3

clone 3D9.3, from mouse

别名:

Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12

登录 查看组织和合同定价。

选择尺寸

变更视图

关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3D9.3, monoclonal
Application:
FACS, ICC, WB
Citations:
3
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助


biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3D9.3, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CASP3(836)

General description

胱天蛋白酶-3(CPP32,Apopain)是研究最广泛的凋亡蛋白。胱天蛋白酶-3被合成为一种无活性的酶(32 kDa),该酶在经历凋亡的细胞中通过自身蛋白水解和/或被另一种上游蛋白酶切割而处理。胱天蛋白酶-3的加工形式由大(17 kDa)和小(12 kDa)亚基组成,它们结合形成活性酶。活性胱天蛋白酶-3蛋白水解切割并激活其他胱天蛋白酶,以及细胞中的相关靶标,例如PARP和DFF。
观测值〜17 kDa。胱天蛋白酶3在凋亡过程中裂解为17 kDa和12 kDa活性形式。

Immunogen

对应于人胱天蛋白酶3(活性形式)的KLH偶联线性肽。

Application

免疫细胞化学分析:代表性批次的1:500稀释液在Hela和Hela/星形孢菌素3小时细胞中检测到胱天蛋白酶3(活性形式)。
流式细胞术分析:代表性批次的1:400稀释液在Jurkat T细胞中检测到胱天蛋白酶3(活性形式)。
抗胱天蛋白酶3(活性形式),克隆3D9.3是一种靶向胱天蛋白酶3(活性形式)蛋白的抗体,经验证可用于WB、ICC&流。

Biochem/physiol Actions

该抗体显示出对裂解的17 kDa活性形式的特异性,但不与35 kDa的非活性(原)形式的胱天蛋白酶3发生交叉反应。

Physical form

形式:纯化

Analysis Note

通过蛋白质印迹在HeLa 细胞裂解物中的进行了评估。

蛋白质印迹分析:0.1µ g/mL该抗体可在10µ g HeLa和星形孢菌素处理的HeLa细胞裂解物中检出胱天蛋白酶3(活性形式)。

Other Notes

替代品:04-1090;04-439
浓度:请参考批次特异性浓缩物的分析证书。


Still not finding the right product?

试用我们的 产品选型工具 工具缩小选择范围


存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库



Catalina Silva-Hirschberg et al.
Therapeutic advances in medical oncology, 11, 1758835919891567-1758835919891567 (2019-12-17)
Mycosis fungoides (MF) and Sézary syndrome (SS) are subtypes of primary cutaneous lymphomas and represent complex diseases regarding their physiopathology and management. Depending on the stage of the disease, different treatment regimens are applied, but there is no consensus on
John Chun-Hao Chen et al.
Oncology letters, 17(1), 638-645 (2019-01-19)
Trichostatin A (TSA), a hydroxamate histone deacetylase inhibitor, is a compound that has been identified to induce anticancer activity. The aim of the present study was to investigate whether sorafenib, in combination with TSA, was able to augment the anticancer
Mohammadhossein Khorraminejad-Shirazi et al.
Stem cell research & therapy, 11(1), 45-45 (2020-02-06)
Mesenchymal stromal cell (MSC) stemness capacity diminishes over prolonged in vitro culture, which negatively affects their application in regenerative medicine. To slow down the senescence of MSCs, here, we have evaluated the in vitro effects of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), an



全球贸易项目编号

货号GTIN
MAB1075304053252850653