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MAB2605

Anti-ATG5 Antibody, clone 177.19

Name: Anti-ATG5 Antibody, clone 177.19
Brand: MM

clone 177.19, from mouse

别名:

APG5 autophagy 5-like, APG5 autophagy 5-like (S. cerevisiae), APG5 (autophagy 5, S. cerevisiae)-like, ATG5 autophagy related 5 homolog (S. cerevisiae), ATG5 autophagy related 5-like, Apoptosis-specific protein

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

177.19, monoclonal

Application:

IP, WB

Citations:

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属性

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

177.19, monoclonal

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NM_004849.2

UniProt accession no.

Q9H1Y0

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATG5(9474)

说明

General description

50 kDa

ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity

Immunogen

Linear Peptide, aa: 8-318

Application

Immunoprecipitation: HeLa cell lysate

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.

Biochem/physiol Actions

Other species have not been tested.

The antibody reacts with the 50 kDa ATG5 protein.

Physical form

Format: Purified

Protein G Purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Western Blot and Immunoprecipitation: HeLa cell lysate.

Western Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABC137

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c