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Merck
CN

MAB3101

Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 5B9.2

clone 5B9.2, Chemicon®, from mouse

别名:

Cx45

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5B9.2, monoclonal
Application:
ICC, WB
Citations:
13
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5B9.2, monoclonal

species reactivity

rat, rabbit, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GJC1(10052)
rat ... Gjc1(266706)

Immunogen

Epitope: near C-terminus, cytoplasmic
The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.

Application

Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 5B9.2 detects level of Connexin 45 & has been published & validated for use in IC & WB.
Immunblotting (~46 kDa)

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Biochem/physiol Actions

Recognizes Human Connexin 45.

SPECIES REACTIVITIES:

Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.

Physical form

Format: Purified
Liquid at 1 mg/mL in 0.02M PB with 0.25 M NaCl, pH=7.6 containing 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

存储类别

12 - Non Combustible Liquids



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Expression of connexins 36, 43, and 45 during postnatal development of the mouse retina.
Alexandre Hiroaki Kihara, Leandro Mantovani de Castro, Monica Aparecida Belmonte et al.
Journal of Neurobiology null
Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1.
Li, X; Kamasawa, N; Ciolofan, C; Olson, CO; Lu, S; Davidson, KG; Yasumura, T; Shigemoto et al.
The Journal of Neuroscience null
Axonal synapses utilize multiple synaptic ribbons in the mammalian retina.
Kim, HL; Jeon, JH; Koo, TH; Lee, UY; Jeong, E; Chun, MH; Moon, JI; Massey, SC; Kim, IB
Testing null



全球贸易项目编号

货号GTIN
MAB310104053252614477