MAB3242
Smoothelin抗体，克隆R4A
clone R4A, Chemicon®, from mouse
别名:
Smoothelin检测抗体, 克隆R4A抗Smoothelin抗体
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
R4A, monoclonal
Application:
ICC, IHC, WB
Citations:
15
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
clone
R4A, monoclonal
species reactivity
pig, chicken, monkey, canine, feline, human, hamster
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SMTN(6525)
General description
Smoothelin是平滑肌细胞（SMC）细胞骨架的组成部分。针对smoothelin的抗体是监测SMC分化的有用工具。Smoothelin仅在完全分化（收缩）的SMC中表达。RNA和蛋白质分析揭示了该蛋白的广泛性物种分布。肌成纤维细胞、肌上皮细胞以及骨骼肌和心肌等具有SMC样特征的细胞不含smoothelin。组织切片的共聚焦扫描激光显微镜以及转染研究显示，smoothelin的丝状组织不同于结蛋白或波形蛋白。smoothelin与肌动蛋白应力纤维共定位。在免疫印迹中检测到两种分子量同工型，即对内脏SMC具有特异性的50 kDa同工型和血管SMC中分子量约为100 kDa的同工型。人smoothelin由位于22号染色体上的单拷贝基因编码。MAB3242可用于区分完全分化的收缩SMC于与增殖SMC。smoothelin阳性细胞的分布表明smoothelin表达与平滑肌组织收缩力之间存在相关性。Smoothelin是评估动脉粥样硬化病变的有用工具。
Immunogen
鸡胗细胞骨架提取物。
Application
可使用这种Smoothelin抗体（克隆R4A）检测Smoothelin，该抗体经验证可用于WB、IC、IH(P)。
蛋白质印迹
免疫细胞化学
冷冻和石蜡包埋的组织切片的免疫组化。对于石蜡切片，强烈建议在10 mM柠檬酸盐缓冲液（pH 6.0）中进行高温抗原修复（van der Loop, 1997）。
最佳工作稀释度必须由最终用户进行确定。
免疫细胞化学
冷冻和石蜡包埋的组织切片的免疫组化。对于石蜡切片，强烈建议在10 mM柠檬酸盐缓冲液（pH 6.0）中进行高温抗原修复（van der Loop, 1997）。
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
MAB3242可与平滑肌细胞中特有的细胞骨架蛋白smoothelin反应。可通过蛋白质印迹法与对应于smoothelin的59 kDa和100 kDa蛋白反应。
Physical form
形式：纯化
液体，溶于含有0.1％叠氮化钠的缓冲液（PBS）中。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Yan Zhang et al.
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology, 19(6), e233-e240 (2010-01-23)
We have recently shown that native murine ventricular fibroblasts express both connexin43 (Cx43) and Cx45, and that the level of Cx43 expression influences intercellular coupling and cell proliferation. Relatively little is known, however, about how myocardial infarction (MI) influences expression
Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype.
Hector F Simosa, Grace Wang, XinXin Sui, Timothy Peterson, Vinod Narra, Dario C Altieri et al.
Journal of Vascular Surgery null
Eric van der Veer et al.
Circulation research, 97(1), 25-34 (2005-06-11)
Conversion of vascular smooth muscle cells (SMCs) from a proliferative state to a nonproliferative, contractile state confers vasomotor function to developing and remodeling blood vessels. Using a maturation-competent human SMC line, we determined that this shift in phenotype was accompanied
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB3242 | 04053252611476 |