MAB4381C3
Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate
clone TRA-1-81, from mouse, CY3 conjugate
别名:
Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
CY3 conjugate
Clone:
TRA-1-81, monoclonal
Application:
ICC
Citations:
11
biological source
mouse
Quality Level
conjugate
CY3 conjugate
antibody form
saturated ammonium sulfate (SAS) precipitated
antibody product type
primary antibodies
clone
TRA-1-81, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PODXL(5420)
General description
420/250 kDa calculated
TRA-1-81 is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). Both TRA-1-81 and TRA-1-60 recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope while TRA-1-81 reacts with an unknown epitope of the same molecule. TRA-1-81 is commonly used as marker of undifferentiated pluripotent human stem cells and is downregulated upon differentiation. TRA-1-81 has recently been identified as podocalyxin.
Immunogen
Human embryonal carcinoma cell line 2102Ep
Application
Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-81 in mouse embryonic stem cells (SCR012).
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate is validated for use in ICC for the detection of TRA-1-81.
Biochem/physiol Actions
This antibody recognizes human TRA-1-81.
Physical form
Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Preparation Note
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
H9 human embryonic stem cells
H9 human embryonic stem cells
Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Still not finding the right product?
试用我们的 产品选型工具 工具缩小选择范围
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
A nonviral minicircle vector for deriving human iPS cells.
Jia, Fangjun, et al.
Nature Methods, 7, 197-199 (2010)
G Badcock et al.
Cancer research, 59(18), 4715-4719 (1999-09-24)
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
Andrews, P W, et al.
Hybridoma, 3, 347-361 (1984)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB4381C3 | 04053252694707 |