Anti-Bax Antibody, NT, aa3-16 hBax protein, clone 2D2

The previously assigned protein identifier Q07814 has been merged into Q07812. Full details can be found on the UniProt database.

Epitope: N-terminus, aa3-16 hBax protein

Synthetic peptide corresponding to aa 3-16 (GSGEQPRGGGPTSS) of human bax. This amino acid sequence is not shared by mouse or rat Bax.

Anti-Bax Antibody, N-terminus, aa3-16 hBax protein, clone 2D2 is an antibody against Bax for use in IF, WB, IH(P).

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

Western blot: 1 μg/ml for 2 hours at RT (1:200 dilution)

Immunofluorescence

Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT

(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT)

Optimal working dilutions must be determined by end user.

Recognizes a 21 kD protein. Does not cross-react with Bcl-2a or Bcl-X. In contrast to Bcl-2a and Bcl-X, overexpression of Bax protein accelerates apoptosis.

Format: Purified

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-434

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