biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3-E7, monoclonal
species reactivity
pig, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... POMC(5443)
Immunogen
Synthetic human beta-endorphin coupled with poly-lysine or thyroglobulin (Gramsch et al., 1983; Meo et al., 1983) (H-Tyr-Gly-Gly-Phe sequence).
Application
ELISA Immunohistochemistry
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Hormones & Receptors
Neuroinflammation & Pain
Hormones & Receptors
This Anti-Endorphin Antibody, β, clone 3-E7 is validated for use in ELISA, IH for the detection of Endorphin.
Biochem/physiol Actions
The antibody reacts with the amino terminal H-Tyr-Gly-Gly-Phe sequence of human O-endorphin. Therefore, a high cross-reactivity with homologues of identical sequence, such as [Met]-enkephalin and [Leu]-enkephalin and a number of opioid peptides, occurs; the antibody also reacts with swine and camel O-endorphin. However, the antibody does not recognize human beta-lipotropin and N-acetyl-beta-endorphin (Gramsch et al., 1983; Meo et al., 1983; Herz et al., 1982).
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
Binding characteristics of a monoclonal beta-endorphin antibody recognizing the N-terminus of opioid peptides.
Gramsch, C, et al.
Journal of Neurochemistry, 40, 1220-1226 (1983)
W H Oertel et al.
Life sciences, 33 Suppl 1, 73-76 (1983-01-01)
Opioid peptide-like (OPL)-immunoreactivity and (the GABA-biosynthetic enzyme) glutamic acid decarboxylase-like (GAD)-immunoreactivity were localized in rat neostriatum and central amygdaloid nucleus (ACE) using a polyclonal sheep antiserum to rat brain GAD and a monoclonal mouse antibody to the N-terminus of beta-endorphin
Characteristics of a monoclonal beta-endorphin antibody recognizing the N-terminus of opioid peptides.
Herz, A, et al.
Life Sciences, 31, 1721-1724 (1982)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB5276 | 04053252617898 |