biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity
sheep, mouse, mule deer, bovine, elk
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PRNP(5621)
Immunogen
Recombinant PrP amino acids 23-237.
Application
Anti-Prion Protein Antibody is an antibody against Prion Protein for use in ELISA, WB, IH.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Western blot: 1:50,000-1:200,000
Immunohistochemistry: 1:100-1:500. Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:
a. formic acid for 10 minutes at room temperature
b. hydrolytic autoclaving
c. heat induced epitope retrieval
ELISA: 1:20,000-1:50,000
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:100-1:500. Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:
a. formic acid for 10 minutes at room temperature
b. hydrolytic autoclaving
c. heat induced epitope retrieval
ELISA: 1:20,000-1:50,000
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with prion protein (PrP) from bovine, sheep, mule deer, elk and mouse. The monoclonal recognizes both protease sensitive and protease resistant forms of PrP (after denaturing).
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS. Contains no preservative.
Preparation Note
Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Christopher J Silva
Prion, 6(2), 163-173 (2012-03-23)
PrP(Sc) is an infectious protein. The only experimentally verified difference between PrP(Sc) and its normal cellular isoform (PrP(C)) is conformational. This work describes an approach to determining the presence of surface exposed or sequestered amino acids present in the PrP(Sc)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB5424 | 04053252467172 |