biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
7F7.1, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NANOG(79923)
General description
NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors.
~39 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human NANOG.
Application
Anti-NANOG Antibody, clone 7F7.1 is an antibody against NANOG for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NANOG in H9 human embryonic stem cells.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Transcription Factors
Transcription Factors
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
H9 human embryonic stem cell lysate
H9 human embryonic stem cell lysate
Evaluated by Western Blot in H9 human embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Still not finding the right product?
试用我们的 产品选型工具 工具缩小选择范围
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.
The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.
Ambrin Fatima et al.
Stem cell research, 44, 101739-101739 (2020-03-04)
Incontinentia pigmenti (IP) is an X-linked dominant neuroectodermal dysplasia caused by loss-of-function mutations in the IKBKG gene. Using CRISPR/Cas9 technology, we generated an IKBKG knock-out iPSC line (KICRi002-A-1) on a 46,XY background. The iPSC line showed a normal karyotype, expressed
Jens Schuster et al.
Stem cell research, 57, 102577-102577 (2021-10-24)
Heterozygous variants in POLR2A, encoding the largest subunit of RNA polymerase II, cause severe neurodevelopmental and multisystem abnormalities in humans. Using CRISPR/Cas9 we generated the human iPSC line KICRi002A-5 with a heterozygous truncating 4 bp insertion in exon 5 of the
Yifan Zhou et al.
Molecular autism, 7, 42-42 (2016-10-08)
Fragile X syndrome (FXS), a common cause of intellectual disability and autism, results from the expansion of a CGG-repeat tract in the 5' untranslated region of the FMR1 gene to >200 repeats. Such expanded alleles, known as full mutation (FM)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MABD24 | 04053252437991 |