Anti-DAX-1, clone 2F4 Antibody

DAX-1 is an orphan nuclear receptor and is required for the normal development of the hypothalamic-pituitary-adrenal-gonadal axis formation. Defects in DAX-1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC); also known as X-linked Addison disease (AHX). DAX-1 also has a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency by acting as a negative regulator of Oct3/4 in ES cells.

~ 52 kDa observed

Linear peptide corresponding to Human DAX-1 (amino acids 135-166).

Anti-DAX-1, clone 2F4, has been shown to work in Immunocytochemical (Lehmann et al., 2009) and Immunohistochemical (Ehrlund et al., 2013) analysis.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-DAX-1, clone 2F4 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of DAX-1.

Mouse monoclonal IgG1κ Ascites with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blot in a Dax-1 overexpressing HEK293 transfected cell line. Western Blot Analysis: A 1:1000 dilution of this antibody detected DAX-1 in a DAX-1 overexpressing HEK293 transfected cell line.

Replaces: 04-445

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.