biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
PC10, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, yeast, mouse, human, zebrafish
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (2.5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (not recommended with unconjugated antibody)
Paraffin Sections (2.5 µg/mL, no pre-treatment required))
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... PCNA(5111)
zebrafish ... Pcna(30678)
General description
识别HeLa细胞以及扁桃体或正常结肠组织中的~37 kDa PCNA蛋白。
该抗PCNA（Ab-1）小鼠mAb（PC10）经验证可用于免疫印迹、免疫荧光、免疫沉淀石蜡切片以检测PCNA（Ab-1）。
通过使用指定的免疫原免疫BALB/c小鼠并将脾细胞与Sp2/0-Ag14小鼠骨髓瘤细胞融合而产生的纯化的小鼠单克隆抗体。识别~37 kDa PCNA蛋白。
Immunogen
表位：氨基酸112-121内
重组PCNA
Application
免疫印迹（2.5 µg/mL）
免疫荧光（1-5 µg/mL）
免疫沉淀（不建议使用未结合的抗体，请参见注释）
石蜡切片（2.5 µg/mL，无需预处理，请参见注释）
免疫荧光（1-5 µg/mL）
免疫沉淀（不建议使用未结合的抗体，请参见注释）
石蜡切片（2.5 µg/mL，无需预处理，请参见注释）
Packaging
批次特定浓度请参见小瓶标签。
Analysis Note
阳性对照
HeLa细胞、HL-60细胞、扁桃体组织或正常结肠组织
HeLa细胞、HL-60细胞、扁桃体组织或正常结肠组织
Other Notes
Waseem, N.H. and Lane, D.P.1990.J. Cell.Sci.96, 121.
Suzuka, I., et al. 1989.Proc.Natl.Acad.Sci. USA86 3189.
Bravo, R., et al. 1987.Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987.J. Cell.Biol.105, 1549.
Miyachi, K., et al. 1987.J. Immunol.121, 2228.
Suzuka, I., et al. 1989.Proc.Natl.Acad.Sci. USA86 3189.
Bravo, R., et al. 1987.Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987.J. Cell.Biol.105, 1549.
Miyachi, K., et al. 1987.J. Immunol.121, 2228.
染色显示出强烈的核模式。不建议用于免疫沉淀，因为在某些样品中会沉淀出约37 kDa的蛋白质，而不是PCNA。通常，石蜡切片染色不需要预处理，但是在某些样品中，可以通过在37°C下用4 N HCl预处理10分钟，然后在水中洗涤3次来增强染色。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NA03-200UG | 04055977227642 |