Anti-p300 Mouse mAb (NM-11)

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.

Recognizes the p300 protein in A431, HeLa, and ML-1 cells.

This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

Epitope: within amino acids 2071-2091 near the CBP C-terminus

Human

recombinant, human p300 protein

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (1-5 µg/ml, heat pre-treatment required)

Please refer to vial label for lot-specific concentration.

In 0.05 M sodium phosphate buffer, 50% glycerol.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
A431, HeLa, or ML-1 cells

Antibody should be titrated for optimal results in individual systems.

Dallas, P.B., et al. 1997. Hybridoma16, 273.
Shisler, J., et al. 1996. J. Virol.70, 68.
Wang, H.G., et al. 1995. J. Virol.69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene9, 1733.
Eckner, R., et al. 1994. Genes Dev.8, 869.
Abraham, S.E., et al. 1993. Oncogene8, 1639.
Wang, H.G., et al. 1993. J. Virol.67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.
Yee, S. and Branton, P., 1985. Virology147, 142.

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Toxicity: Standard Handling (A)