biological source
mouse
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
162 33 D3, monoclonal
form
liquid
does not contain
preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (0.1 µg/mL)
Flow Cytometry (1 µg/mL)
Frozen Sections (5-10 µg/mL)
Immunoblotting (5 µg/mL)
Immunofluorescence (1 µg/mL)
Paraffin Sections (5-10 µg/mL)
Radioimmunoassay (0.1 µg/mL)
Southwestern Blot (5 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
免疫亲和纯化的小鼠单克隆抗体。识别5-甲基胞嘧啶。
经验证，该抗5-甲基胞嘧啶小鼠mAb（162 33 D3）可用于ELISA、FC、冷冻切片、IF、石蜡切片、放射免疫分析、DNA-蛋白质印迹法检测5-甲基胞嘧啶。
识别NIH3T3细胞中甲基化DNA或RNA中的5-甲基胞嘧啶。
特异性
•亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
• 流式细胞术
•冷冻切片
• 免疫印迹
•免疫荧光
•石蜡切片
可靠
•产生可重复的结果。
•克隆162 33 D3已在50多个出版物中使用。
特异性
•亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
• 流式细胞术
•冷冻切片
• 免疫印迹
•免疫荧光
•石蜡切片
可靠
•产生可重复的结果。
•克隆162 33 D3已在50多个出版物中使用。
Immunogen
5-甲基胞嘧啶结合卵清蛋白
Application
ELISA（0.1 g/mL）
流式细胞术（1 g/mL）
冷冻切片（5-10 g/mL）
免疫印迹法（5 g/mL）
免疫荧光（1 g/mL）
石蜡切片（5-10 g/mL）
放射免疫分析（0.1 g/mL）
DNA-蛋白质印迹法（5 g/mL）
Packaging
批次特定浓度请参见小瓶标签。
Physical form
在PBS（pH 7.4）中。
Preparation Note
初次解冻后，等分并冷冻（-20°C）。
Analysis Note
阳性对照
NIH3T3细胞
NIH3T3细胞
Other Notes
Ehrlich, M. 2002.Oncogene21, 5400.(Review)
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
克隆162 33 D3可用于定量和定性检测各种样品和应用中的甲基化DNA或RNA。它已在40多个出版物中使用。应滴定抗体，以在单个系统中获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NA81-50UG | 04055977209792 |