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NR07

Anti-Ryanodine Receptor Mouse mAb (C3-33)

Name: Anti-Ryanodine Receptor Mouse mAb (C3-33)
Brand: MM

liquid, clone C3-33, Calbiochem^®

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关于此项目

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

C3-33, monoclonal

Species reactivity:

fish, canine, rat, avian, amphibian, rabbit

Application:

—

Citations:

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属性

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

C3-33, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

fish, canine, rat, avian, amphibian, rabbit

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Frozen Sections (1 µg/mL,
Immunoblotting (1 µg/mL)
Immunoprecipitation )

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Ryr2(689560)

说明

General description

Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X 63 AG8.653 mouse myeloma cells (see application references). Supplied as protein G purified antibody. Recognizes the ~565 kDa ryanodine receptor.

Recognizes the ~565 kDa ryanodine receptor in rat brain hippocampus. A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates.

This Anti-Ryanodine Receptor Mouse mAb (C3-33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation for the detection of Ryanodine Receptor.

Immunogen

Canine

canine cardiac ryanodine receptor (RYR)

Application

Frozen Sections (1 g/ml, see application references)
Immunoblotting (1 g/ml)
Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Rat brain hippocampus

Other Notes

A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.

Sitsapesan, R., et al. 1995. Trends Pharmacol. Sci.16, 386.
McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem.268, 13765.
Meszaros, L.G., et al. 1993. Nature364, 76.
Lai, F.A., et al. 1992. Biochem. J.288, 553.
Lai, F.A., et al. 1989. Nature331, 315.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c