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OP03

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

Name: Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)
Brand: MM

liquid, clone PAb421, Calbiochem^®

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关于此项目

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

PAb421, monoclonal

Species reactivity:

rabbit, monkey, human, mouse, rat

Application:

—

Citations:

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属性

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

PAb421, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rabbit, monkey, human, mouse, rat

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

dilution

(Frozen Sections (10 µg/mL,
Gel Shift
Immunoblotting (10 µg/mL,
Immunofluorescence (1-20 µg/mL,
Flow Cytometry (1-20 µg/mL)
Immunoprecipitation (1g per sample, )

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

说明

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.

Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.

This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).

Immunogen

Epitope: within amino acids 376-378 of human p53

Mouse

partially purified mouse p53

Application

Frozen Sections (10 µg/ml, see application references)

Gel Shift (see comments)

Immunoblotting (10 µg/ml, see application references)

Immunofluorescence (1-20 µg/ml, see application references)

Flow Cytometry (1-20 µg/ml)

Immunoprecipitation (1 μg per sample, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Negative Control
SK-OV-3 cells or normal skin

Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)

Other Notes

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.

Originally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with ³⁵S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser³⁷⁶ to Ser³⁷⁸ (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c