OP33
抗-P53（Ab-5）（野生型）小鼠mAb（PAb1620）
liquid, clone PAb1620, Calbiochem®
别名:
p53检测抗体, 小鼠抗p53抗体, 抗p53抗体
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
PAb1620, monoclonal
Species reactivity:
human, mouse, rat, primate, bovine
Application:
—
Citations:
6
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
PAb1620, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, rat, primate, bovine
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (see applications references)
Immunoblotting (not recommended)
Immunofluorescence (1-20 μg/mL)
Immunoprecipitation (1 μg per sample)
Paraffin Sections (5 μg/mL, heat pre-treatment required))
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... TP53(7157)
General description
可识别Hs27细胞和乳腺癌组织中天然构象的~53 kDa野生型p53蛋白。不能识别突变或变性的p53蛋白。
该抗p53（Ab-5）（野生型）小鼠mAb（PAb1620）经验证可用于冰冻切片、免疫印迹、IF、IP和石蜡切片，以检测p53（Ab-5）（野生型）。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与SP2/0 Ag14骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体。可识别~53 kDa野生型p53蛋白。
Immunogen
vLM 小鼠肿瘤细胞
Application
冰冻切片（见应用参考文献）
免疫印迹（不推荐）
免疫荧光（1-20 μg/ml）
免疫沉淀（1 μg/样品）
石蜡切片（5 μg/ml，需要加热预处理）
免疫印迹（不推荐）
免疫荧光（1-20 μg/ml）
免疫沉淀（1 μg/样品）
石蜡切片（5 μg/ml，需要加热预处理）
Packaging
请参考特定浓度批号的标签。
Analysis Note
阳性对照
Hs27细胞或乳腺癌组织
Hs27细胞或乳腺癌组织
Other Notes
El-Deiry, W.S., et al. 1994.Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54, 4855.
Barak, Y., et al. 1993.EMBO J.12, 461.
Kastan, M.B., et al. 1992.Cell71, 587.
Kuerbitz, S.J.1992.Proc.Natl.Acad.Sci. USA89, 7491.
Lane, D.P.1992.Nature358, 15.
Kastan, M.B., et al. 1991.Cancer Res.51, 6304.
Ball, R.K., et al. 1984.EMBO J.3, 1485.
Greenblatt, M.S., et al. 1994.Cancer Res.54, 4855.
Barak, Y., et al. 1993.EMBO J.12, 461.
Kastan, M.B., et al. 1992.Cell71, 587.
Kuerbitz, S.J.1992.Proc.Natl.Acad.Sci. USA89, 7491.
Lane, D.P.1992.Nature358, 15.
Kastan, M.B., et al. 1991.Cancer Res.51, 6304.
Ball, R.K., et al. 1984.EMBO J.3, 1485.
野生型p53的半衰期很短，并且在细胞中的含量很低。增加要免疫沉淀并施加到凝胶上的样品量可能有助于可视化。与35S-Met的短孵育时间（≤1小时）将有助于降低背景。p53（Ab-5）也可用于使用标准辣根过氧化物酶或免疫荧光检测技术观察细胞纺丝或培养细胞中的p53。p53（Ab-5）优先免疫沉淀野生型p53；它不应沉淀突变或变性的p53。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| OP33-100UG | 04055977208443 |
| OP33-20UG | 07790788053901 |