biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
RAS 10, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Flow Cytometry (2.5 µg/mL)
Frozen Sections (2.5 µg/mL)
Immunoblotting (0.1-3 µg/mL, chemiluminescent and colorimetric,
Immunofluorescence (2.5 µg/mL,
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2.5 µg/mL, saponin pre-treatment required))
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... KRAS(3845)
General description
可识别SW480和Y1细胞以及乳腺癌组织中的~21 kDa H-、K-和N-Ras蛋白。
该抗Pan-Ras（Ab-3）小鼠mAb（RAS 10）经验证可用于FC、冰冻切片、免疫印迹、IF、IP和石蜡切片，以检测Pan-Ras（Ab-3）。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与SP2/0骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体（请参阅应用参考）。可识别~21 kDa Ras蛋白。
Immunogen
重组人p21 Ras
Application
流式细胞术分析（2.5 g/ml）
冷冻切片（2.5 g/ml）
免疫印迹（0.1-3 g/ml，化学发光和比色法，请参阅应用参考文献）
免疫荧光（2.5 g/ml，请参阅应用参考文献）
免疫沉淀（1 g/样品）
石蜡切片（2.5 g/ml，需要皂苷预处理）
Packaging
请参考特定浓度批号的标签。
Analysis Note
阳性对照
SW480或Y1细胞或乳腺癌组织
SW480或Y1细胞或乳腺癌组织
Other Notes
Rodenhuis, S. et al., 1992.Cancer Res. (Suppl)52, 2665s.
Sidransky, D., et al. 1992.Science256, 102.
Hamer, P.J., et al. 1990.Hybridoma9, 573.
Bos, J.L.1989.Cancer Res.49, 4682.
Furth, M. E., et al. 1987.Oncogene1, 47.
Kraus, M.H., et al. 1984.Proc.Natl.Acad.Sci. USA81, 5384.
Shimizu, K., et al. 1983.Proc.Natl.Acad.Sci. USA80, 2112.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.
Sidransky, D., et al. 1992.Science256, 102.
Hamer, P.J., et al. 1990.Hybridoma9, 573.
Bos, J.L.1989.Cancer Res.49, 4682.
Furth, M. E., et al. 1987.Oncogene1, 47.
Kraus, M.H., et al. 1984.Proc.Natl.Acad.Sci. USA81, 5384.
Shimizu, K., et al. 1983.Proc.Natl.Acad.Sci. USA80, 2112.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.
p21ras的表达水平在不同组织中是可变的。因此，我们建议在免疫印迹分析之前进行浓缩步骤，以获得最佳结果。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Lynn B Eckert et al.
Cancer research, 64(13), 4585-4592 (2004-07-03)
Although mutated forms of ras are not associated with the majority of breast cancers (<5%), there is considerable experimental evidence that hyperactive Ras can promote breast cancer growth and development. Therefore, we determined whether Ras and Ras-responsive signaling pathways were
Elzbieta Janda et al.
Oncogene, 21(33), 5148-5159 (2002-07-26)
Carcinogenesis by oncogenic Ras and Her-2 involves enhanced proliferation of epithelial cells in vivo. However, hyperproliferation induced by these oncogenes, or their downstream pathways in vitro has mainly been studied in cultured, fibroblastic cell lines. Here, we demonstrate that oncogenic
Junko Sonoyama et al.
The Journal of biological chemistry, 277(10), 8076-8082 (2002-01-10)
BCR/ABL tyrosine kinase generated from the chromosomal translocation t(9;22) causes chronic myelogenous leukemia and acute lymphoblastic leukemia. To examine the roles of BCR/ABL-activated individual signaling molecules and their cooperation in leukemogenesis, we inducibly expressed a dominant negative (DN) form of
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| OP40-100UG | 07790788054458 |