biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2B, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (2 µg/mL)
Immunoprecipitation (2 µg))
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BRCA2(675)
General description
可识别表达野生型BRCA2基因的细胞中的~460 kDa BRCA2蛋白。根据美国专利号5,753,441和6,162,897的许可证销售。
抗BRCA2（Ab-1），小鼠单克隆，克隆2B，可识别表达野生型BRCA2基因的细胞中的~460 kDa BRCA2蛋白。它已被验证可用于蛋白质印迹和免疫沉淀。
通过用指定的免疫原免疫小鼠并将脾细胞与骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体。可识别~460 kDa BRCA2蛋白。
Immunogen
人
由人BRCA2的氨基酸1651-1821与GST融合而成的重组蛋白
Application
免疫印迹法（2 µg/ml）
免疫沉淀（2 µg）
免疫沉淀（2 µg）
Packaging
请参考特定浓度批号的标签。
Physical form
溶于PBS。
Preparation Note
初次融化后，等分并冷冻（-20°C）。
Analysis Note
阳性对照
MCF7细胞
MCF7细胞
Other Notes
Andres, J.L., et al. 1998.Oncogene16, 2229.
Chen, J., et al. 1998.Mol.Cell2, 317.
Chen, P.L., et al. 1998.Proc.Natl.Acad.Sci. USA95, 5287.
Marmorstein, L.Y., et al. 1998.Proc.Natl.Acad.Sci. USA95, 13869.
Connor, F., et al. 1997.Nat. Genet.17, 423.
Sharan, S.K., et al. 1997.Nature386, 804.
Ludwig, T., et al. 1997.Genes Dev.11, 1226.
Mizuta, R., et al. 1997.Proc.Natl.Acad.Sci. USA94, 6927.
Rajan, J.V., et al. 1997.Dev.Biol.184, 385.
Suzuki, A., et al. 1997.Genes Dev.11, 1242.
Wong, A.K.C., et al. 1997.J. Biol. Chem.272, 31941.
Cannon-Albright, L.A. and M.H.Skolnick.1996.Semin.Oncol.23, 1.
Goggins, M., et al. 1996.Cancer Res.56, 5360.
Stratton, M.R.1996.Hum.Mol.Genet.5, 1515.
Tavtigian, S.V., et al. 1996.Nat. Genet.12, 333.
Rajan, J.V., et al. 1996.Proc.Natl.Acad.Sci. USA93, 13078.
Spillman, M.A. and A.M. Bowcock.1996.Oncogene13, 1639.
Vaughn, J.P., et al. 1996.Cancer Res.56, 4590.
Wooster, R., et al. 1995.Nature378, 789.
Chen, J., et al. 1998.Mol.Cell2, 317.
Chen, P.L., et al. 1998.Proc.Natl.Acad.Sci. USA95, 5287.
Marmorstein, L.Y., et al. 1998.Proc.Natl.Acad.Sci. USA95, 13869.
Connor, F., et al. 1997.Nat. Genet.17, 423.
Sharan, S.K., et al. 1997.Nature386, 804.
Ludwig, T., et al. 1997.Genes Dev.11, 1226.
Mizuta, R., et al. 1997.Proc.Natl.Acad.Sci. USA94, 6927.
Rajan, J.V., et al. 1997.Dev.Biol.184, 385.
Suzuki, A., et al. 1997.Genes Dev.11, 1242.
Wong, A.K.C., et al. 1997.J. Biol. Chem.272, 31941.
Cannon-Albright, L.A. and M.H.Skolnick.1996.Semin.Oncol.23, 1.
Goggins, M., et al. 1996.Cancer Res.56, 5360.
Stratton, M.R.1996.Hum.Mol.Genet.5, 1515.
Tavtigian, S.V., et al. 1996.Nat. Genet.12, 333.
Rajan, J.V., et al. 1996.Proc.Natl.Acad.Sci. USA93, 13078.
Spillman, M.A. and A.M. Bowcock.1996.Oncogene13, 1639.
Vaughn, J.P., et al. 1996.Cancer Res.56, 4590.
Wooster, R., et al. 1995.Nature378, 789.
在单个BRCA2等位基因中具有6174delT突变的CAPAN-1细胞系表达230 kDa的截短的BRCA2蛋白，该蛋白包含抗BRCA2表位并保留RAD51结合但失去了p53结合。全长BRCA2蛋白将在SDS-PAGE上与DNA PK的催化亚基共迁移，可以使用抗DNA PK（目录编号PC127）检测。在单个系统中，应对抗体进行滴定以获得最佳结果。
Legal Information
根据美国专利号5,837,492和6,124,104的许可证销售。
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
M Goggins et al.
Cancer research, 56(23), 5360-5364 (1996-12-01)
Germline mutations in BRCA2 predispose carriers to the development of breast, ovarian, and a variety of other cancers. The original localization of the BRCA2 gene was aided by its homozygous deletion in a pancreatic carcinoma; indeed, an excess of pancreatic
F Connor et al.
Nature genetics, 17(4), 423-430 (1997-12-17)
Germline mutation of the BRCA2 gene carries a high risk of developing breast cancer. To study the function of this gene, we generated a mutation in Brca2 in mice. Unlike other mutations in the Brca2 gene, which are lethal early
R Mizuta et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(13), 6927-6932 (1997-06-24)
The human RAD51 protein is a homologue of the bacteria RecA and yeast RAD51 proteins that are involved in homologous recombination and DNA repair. RAD51 interacts with proteins involved in recombination and also with tumor suppressor proteins p53 and breast
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| OP95-100UG | 04055977209709 |