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Merck
CN

SCC008

ReNcell VM人神经祖细胞系

ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

别名:

Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells

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关于此项目

UNSPSC Code:
41106509
NACRES:
NA.81
eCl@ss:
32011203
Biological source:
human
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biological source

human

Quality Level

manufacturer/tradename

Chemicon®, ReNcell

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

liquid nitrogen

storage temp.

-140 to -196°C

General description

ReNcell VM是一种永生化的人神经祖细胞系,具有易于分化为神经元和神经胶质细胞的能力。 ReNcell VM源自人胎脑的腹侧中脑区域。 通过用v-myc癌基因进行逆转录病毒转导而永生化,该细胞系在层粘连蛋白上以单层快速生长,倍增时间为20-30小时。 核型分析表明,即使经过长时间传代(>45代),ReNcell VM仍可在培养物中保留正常的二倍体核型。 ReNcell VM由ReNeuron Group plc开发,ReNeuron Group plc是一家专门将人体体干细胞用于治疗的生物技术公司。 在ReNeuron Group plc进行的实验中,ReNcell VM可以在体外分化为高水平的人多巴胺能神经元。此外,已证明从ReNcell VM分化的神经元具有电生理活性。 ReNcell VM可用于多种研究应用,例如神经毒性、神经发生、电生理学、神经递质和受体功能的研究。 ReNcell Vm细胞是按照法律和伦理的方式获得的,符合现行的当地知情同意程序。

ReNcell现在用于阿尔茨海默′病的3D细胞模型!
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease.Nature.2014 Nov 13;515(7526):274-8.
发表在Nature上
产品来源:人腹侧中脑组织

Application

研究类别
干细胞研究

Biochem/physiol Actions

神经干细胞

Preparation Note

当在推荐的储存条件(液氮)下储存时,ReNcell VM细胞在有效期内保持稳定。 请勿暴露于高温下。 到期后,请丢弃所有剩余的试剂。 我们建议在十代内使用细胞。

Analysis Note

已经验证了每批ReNcell VM细胞的巢蛋白和Sox 2的高水平表达及其自我更新和多谱系分化能力。通过染色体扩散评估,细胞还显示出正常的核型,并且支原体测试为阴性。已对孕妇血液进行了HIV、HTLV、乙型和丙型肝炎筛查。

Other Notes

来自人腹侧中脑组织的> 1x106个活ReNcell VM细胞,冷冻保存。 储存在液氮中。
根据产品文件中详述的“学术使用协议”的条款,本产品预期仅用于销售和销售给学术机构,以供内部学术研究使用。有关任何其他用途的信息,请联系licensing@emdmillipore.com。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

仅供研究使用在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。


存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

法规信息

高风险级别生物产品-Merck

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实验方案

Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

商品

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

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相关内容

ReNcell® VM and ReNcell® CX are two well-established neural stem cell lines derived from developing human brains. ReNcell® VM and CX cells are generated from the ventral mesencephalon and cortical regions of the brain, respectively, and transduced with the myc transcription factor. Both cell lines offer phenotype and genotype stability, in addition to the multipotential neuronal differentiation capacity, over long-term culture. This article describes the characteristics and differentiation of ReNcell® lines. Results of calcium and membrane potential changes in response to various ligands are also shown. The convenience to maintain in culture and flexibility to differentiate to individual scientists’ needs make ReNcell® lines the ideal platform for research and discovery.


Biljana Stangeland et al.
Oncotarget, 6(28), 26192-26215 (2015-08-22)
Glioblastoma (GBM) is both the most common and the most lethal primary brain tumor. It is thought that GBM stem cells (GSCs) are critically important in resistance to therapy. Therefore, there is a strong rationale to target these cells in
A three-dimensional human neural cell culture model of Alzheimer's disease.
Choi, SH; Kim, YH; Hebisch, M; Sliwinski, C; Lee, S; D'Avanzo, C; Chen, H; Hooli et al.
Nature null
A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.
Song, Y; Subramanian, K; Berberich, MJ; Rodriguez, S; Latorre, IJ; Luria, CM; Everley et al.
Scientific data null



全球贸易项目编号

货号GTIN
SCC00804053252662713