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SCM075

ROCK 抑制剂（Y-27632）

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

别名:

Y-27632

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801
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Quality Level

100

form

liquid

mol wt

320.26 g/mol

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

-10 to -25°C

General description

ROCK抑制剂（Y-27632）是包括p160Rock（Ki=140nM）和Rock-II在内的Rho相关蛋白激酶（ROCK）的一种选择性ATP竞争性抑制剂。还可作为肌球蛋白磷酸化和平滑肌收缩的激动剂诱导的Ca2+敏化的一种有效抑制剂。用10uM Y-27632处理一小时后可阻断已发生解离的培养的人类胚胎干细胞（hESC）的凋亡，从而在不影响多能性的情况下提高hESC的存活率和克隆效率1。近期有报告表明Y-27632和Accutase（货号SCR005）所组成的消化酶混合配方，能够通过使用常规的慢速冷冻和快速解冻方法提高单个hESC的解冻后存活率

Preparation Note

在重悬前：维持在-20ºC避光保存2年。在重悬后：在-20ºC避光保存1年

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

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实验方案

通过无血清细胞培养条件将人多能诱导干细胞(iPS)体外分化为结肠类器官

高度表征的冻存人结肠类器官，iPS细胞分化为肠道上皮类器官的分步方案。

Forskolin-Induced Swelling Assays using Millicell® Microwell Plates

Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

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商品

Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

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全球贸易项目编号

货号GTIN
SCM07504053252374654