SF006
ESGRO Complete 细胞消化液
The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.
别名:
Stem Cell Tested Accutase
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关于此项目
UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801
Quality Level
form
liquid
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
shipped in
dry ice
storage temp.
-10 to -25°C
General description
ESGRO Complete™ Accutase 是一种蛋白水解和胶原分解酶的细胞分离溶液，经确认可用于分离在无血清条件下用ESGRO Complete 克隆级培养基（目录号SF001-500）培养的小鼠胚胎干细胞。 Accutase 不含有哺乳动物或细菌来源产品。
Application
细胞分离：
1.将Accutase®在室温下解冻。
2.用无菌PBS洗涤孔板、烧瓶或珠子。
3.通过无菌程序添加Accutase至培养皿或烧瓶中（每75平方厘米表面积10 mL）。
4.经培养物放回37°C培养箱中，以分离细胞（5-10分钟）。
5.按常规操作进行细胞计数和传代。不需要额外的洗涤或酶抑制剂。
1.将Accutase®在室温下解冻。
2.用无菌PBS洗涤孔板、烧瓶或珠子。
3.通过无菌程序添加Accutase至培养皿或烧瓶中（每75平方厘米表面积10 mL）。
4.经培养物放回37°C培养箱中，以分离细胞（5-10分钟）。
5.按常规操作进行细胞计数和传代。不需要额外的洗涤或酶抑制剂。
Physical form
冷冻无菌液体，即用型制剂。 每批都已经过无菌性（通过USP膜过滤方法）、酶活性（通过合成发色四肽测试）和细胞与组织培养塑料的分离性能测试。
1X accutase® 酶，溶于含0.5 mM EDTA•4Na和3 mg/L苯酚红的Dulbecco′s PBS中。
1X accutase® 酶，溶于含0.5 mM EDTA•4Na和3 mg/L苯酚红的Dulbecco′s PBS中。
Preparation Note
保存于-20°C可保持稳定。请参阅标签上的批次失效日期。 建议收货时保存于-20°C。 解冻后，Accutase® 可以在4°C下最多保存2个月。请勿在室温下存放。
Legal Information
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Thangaselvam Muthusamy et al.
Stem cell reports, 3(1), 169-184 (2014-07-30)
We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450-500 nm) is readily observed by fluorescence microscopy and correlates with the expression of
Na Suo et al.
Glia, 67(7), 1320-1332 (2019-03-01)
Oligodendrocytes (OLs) are the myelinating glia of the central nervous system. Injury to OLs causes myelin loss. In demyelinating diseases, such as multiple sclerosis, the remyelination is hindered principally due to a failure of the oligodendrocyte precursor cells (OPCs) to
Gabi Tremml et al.
Current protocols in stem cell biology, Chapter 1, Unit 1C-Unit 1C (2008-09-05)
In this unit standard culture conditions for mouse embryonic stem cells (mESCs) on primary murine embryonic fibroblast (PMEF or MEF) monolayers, culture conditions without MEF for feeder-independent mESCs, and culture conditions in chemically defined media for both feeder-independent mESCs and
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SF006 | 04053252503061 |