biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (1:2000)
Immunoprecipitation (1:100))
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~79-85 kDa TORC2 protein.
Recognizes the ~79-85 kDa TORC2 protein in primary hepatocytes, HepG2, NIH3T3, Min6, and FAO cells.
TORCs (Transducers Of Regulated cAMP Response Element-Binding (CREB) protein) are members of a conserved family of co-activators that enhance CRE-dependent transcription by a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB. Studies suggest that TORC phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP.
This Anti-TORC2 (454-607) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of TORC2 (454-607).
Immunogen
Human
a recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
Application
Immunoblotting (1:2000, see comments)
Immunoprecipitation (1:100, see comments)
Physical form
Undiluted serum.
Preparation Note
For long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
Analysis Note
Positive Control
Primary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
Primary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
Other Notes
Screaton, R.A., et al. 2004. Cell119, 61.
This antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
null
John Le Lay et al.
Cell metabolism, 10(1), 55-62 (2009-07-09)
The liver contributes to glucose homeostasis by promoting either storage or production of glucose, depending on the physiological state. The cAMP response element-binding protein (CREB) is a principal regulator of genes involved in coordinating the hepatic response to fasting, but
Rong Wu et al.
Cell metabolism, 29(3), 653-667 (2018-12-12)
Although emerging evidence indicates an important role of the circadian clock in modulating the diurnal oscillation of mammalian target of rapamycin complex 1 (mTORC1) signaling, the underlying molecular mechanism remains elusive. Here we show that Period2 (Per2), a core clock
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| ST1099-50UL | 04055977224436 |