biological source
human plasma
form
lyophilized
specific activity
~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)
mol wt
Mr ~33.6 kDa
packaging
pkg of 20 U
manufacturer/tradename
Roche
optimum pH
8.2-9.0
shipped in
wet ice
General description
凝血酶是一种Na+变构丝氨酸蛋白酶。它属于糜蛋白酶家族。凝血酶是由两条多肽链组成，一条含有36个氨基酸残基（A链），另一条有259个残基（B链），两条链通过二硫键共价连接。
凝血酶是凝血因子IIa。它是一种丝氨酸内肽酶，水解肽链和酯键，尤其是在Arg羧基侧的。该酶将纤维蛋白原转化为纤维蛋白。产品含有EDTA和额外的稳定剂。
Application
凝血酶可用于重组RELM（抵抗素样分子家族成员）蛋白的表达和纯化。它被用作血小板在纤维蛋白原 和免疫染色分散。
凝血酶用于凝血研究，医学研究，蛋白质结构分析和生化研究。它被用作血小板刺激物。
Biochem/physiol Actions
凝血酶在凝血中有很重要的作用。它作为促凝血因子，能够将纤维蛋白原转化成不能溶解的纤维蛋白结块。通过激活蛋白质C，凝血酶可用作抗凝血剂。
Physical form
冻干粉
Preparation Note
储存条件（工作液）：-15至-25℃
抑制剂: DFP, TLCK, PMSF,苯甲脒，α1抗胰蛋白酶α2巨球蛋白，抗凝血酶III，肝素，水蛭素和APMSF
抑制剂: DFP, TLCK, PMSF,苯甲脒，α1抗胰蛋白酶α2巨球蛋白，抗凝血酶III，肝素，水蛭素和APMSF
储存温度2°C至8°C。 （干燥储存！）
重组溶液包含20mM EDTA。
注意：
使用塑料管和枪头因为凝血酶会被玻璃表面吸附！
注意：
使用塑料管和枪头因为凝血酶会被玻璃表面吸附！
Other Notes
仅用于生命科学研究。不可用于诊断。
Disclaimer
仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - STOT RE 2
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
高风险级别生物产品--人源产品
此项目有
M W Mosesson
Journal of thrombosis and haemostasis : JTH, 3(8), 1894-1904 (2005-08-17)
Fibrinogen molecules are comprised of two sets of disulfide-bridged Aalpha-, Bbeta-, and gamma-chains. Each molecule contains two outer D domains connected to a central E domain by a coiled-coil segment. Fibrin is formed after thrombin cleavage of fibrinopeptide A (FPA)
Scott Kaatz et al.
American journal of hematology, 87 Suppl 1, S141-S145 (2012-04-05)
The new oral anticoagulants dabigatran, rivaroxaban and apixaban have advantages over warfarin which include no need for laboratory monitoring, less drug-drug interactions and less food-drug interactions. However, there is no established antidote for patients who are bleeding or require emergent
W Bode et al.
Protein science : a publication of the Protein Society, 1(4), 426-471 (1992-04-01)
Thrombin is a multifunctional serine proteinase that plays a key role in coagulation while exhibiting several other key cellular bioregulatory functions. The X-ray crystal structure of human alpha-thrombin was determined in its complex with the specific thrombin inhibitor D-Phe-Pro-Arg chloromethylketone
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 10602400001 | 04061838673602 |