assay
>99% (titration)
form
solid
packaging
pkg of 500 g
manufacturer/tradename
Roche
technique(s)
FISH: suitable
pH
7.0-9.0
useful pH range
7.0-9.0
pKa (25 °C)
8.1
absorption
<0.02 at 300 nm at 100 mg/mL
storage temp.
room temp
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
Application
孵育混合物中的缓冲试剂。它也被用作裂解缓冲液及TE（Tris-EDTA）缓冲液的一种组分。
Features and Benefits
晶体
Analysis Note
纯度：>99% Tris-HCl（滴定法）
重金属：<1 ppm Pb和Fe
重金属：<1 ppm Pb和Fe
Other Notes
所有缓冲液的pH值都与温度和浓度有关。对于 Tris 缓冲液，温度每降低 1°C，pH 就会增加约 0.03 单位，每稀释 10 倍，pH 会降低 0.03-0.05 单位。对于一些精准的应用，请使用经过仔细校准的pH计和玻璃/甘汞组合电极。
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
MNase Digestion for Nucleosome Mapping in Neurospora.
Cigdem S
Bio-protocol null
Izabela Jęśkowiak et al.
International journal of molecular sciences, 22(20) (2021-10-24)
(1) Background: Melanoma is an aggressive neoplasm derived from melanocyte precursors with a high metastatic potential. Responses to chemotherapy and immunotherapy for melanoma remain weak, underlining the urgent need to develop new therapeutic strategies for the treatment of melanoma. (2)
Sen Wu et al.
Nature protocols, 3(6), 1056-1076 (2008-06-13)
We describe here a streamlined procedure for targeting vector construction, which often is a limiting factor for gene targeting (knockout) technology. This procedure combines various highly efficient recombination-based cloning methods in bacteria, consisting of three steps. First step is the
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 10812846001 | 04061838678157 |