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关于此项目
UNSPSC Code:
12352207
MDL number:
Biological source:
human plasma
Form:
lyophilized (clear, colorless solution after reconstitution)
Technique(s):
cell culture | mammalian: suitable
Mol wt:
440 kDa
Assay:
95% (SDS-PAGE)
biological source
human plasma
sterility
non-sterile; 0.2 μm filtered
assay
95% (SDS-PAGE)
form
lyophilized (clear, colorless solution after reconstitution)
mol wt
440 kDa
packaging
pkg of 5 mg
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... FN1(2335)
General description
纤连蛋白负责细胞与细胞外基质的相互作用。参与细胞的黏附、迁移、生长和分化。是血浆和其他体液的可溶性成分。此外,它也是不溶性细胞外基质的一部分。
Application
促进 3T3 (NR6) 细胞粘附的检测(XTT 切割)
纤维连接蛋白促进许多细胞的附着和随后的扩散。用于培养皿的包被。
Biochem/physiol Actions
对大多数哺乳动物细胞有活性
Physical form
在冻干过滤通过 0.2μm 孔径的膜之前,冷冻干燥物(每 mg 纤连蛋白含有 1.126mg 甘氨酸和 0.058mg 氯化钠)。
Preparation Note
工作浓度: 5μg/cm 2
5μg/cm 2 对于细胞培养容器的包衣
储存条件(工作液):-15°C 至-25°C
复溶溶液在-15°C 至-25°C 下稳定。
注意: 将复溶溶液分等份储存于-15 至-25°C 条件下。
避免反复冻融。
5μg/cm 2 对于细胞培养容器的包衣
储存条件(工作液):-15°C 至-25°C
复溶溶液在-15°C 至-25°C 下稳定。
注意: 将复溶溶液分等份储存于-15 至-25°C 条件下。
避免反复冻融。
分别加入 1 或 5 mL 无菌水,得到最终浓度为 1 mg/mL 的溶液。在 37°C 下孵育 30 至 60 min 以溶解。 不要搅拌。
复溶后,溶液可能含有少量不溶性聚集物。这些聚集体是纤连蛋白固有的现象,不影响产品性能。
复溶后,溶液可能含有少量不溶性聚集物。这些聚集体是纤连蛋白固有的现象,不影响产品性能。
Other Notes
仅用于生命科学研究。不可用于诊断。
Disclaimer
仅供研究使用。在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
高风险级别生物产品--人源产品
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