biological source
sheep
conjugate
alkaline phosphatase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
solution
packaging
pkg of 150 U (200 μl)
manufacturer/tradename
Roche
isotype
IgG
storage temp.
2-8°C
General description
荧光素是由m-二烷基氨基酚或间苯二酚与邻苯二甲酸酐在苛刻条件下缩合而成。Fab 片段与 G 蛋白结合。
该基因编码 SOX (srr-related HMG—box) 转录因子家族的一个成员,参与胚胎发育的调控和细胞命运的决定。编码的蛋白与其他蛋白形成蛋白复合物后可能作为转录激活因子。该蛋白作为核质穿梭蛋白,对神经嵴和周围神经系统发育具有重要意义。该基因的突变与Waardenburg-Shah和Waardenburg-Hirschsprung病有关。(由Ref Seq提供)
Application
使用抗荧光素-AP,Fab片段检测荧光素标记化合物:
- 斑点印迹法
- 免疫分析
- 免疫组织细胞化学
- 原位杂交法
- Southern 印迹法
- Western 印迹法
- 电化学生物传感技术检测 16S rRNA
Biochem/physiol Actions
抗荧光素-AP,Fab 片段已用于寡核苷酸捕获、变性和检测 (CDD)。
多克隆抗体与游离和结合的荧光素反应。
Features and Benefits
目录
溶液,稳定
溶液,稳定
Preparation Note
- 工作浓度: 以下浓度取决于应用和底物,应作为指导。斑点印迹法:150mU/ml
- 免疫分析:150~300mU/ml
- 免疫组织细胞化学:250~500mU/ml
- 原位杂交法:1.5~7.5U/mL
- Southern 印迹法:150mU/ml
- Western 印迹法:250~500mu/ml
工作溶液:100mM Tris-HCl,150mM NaCl,pH 值为 7.5。必要时可用 1% 封闭试剂 (w/v)、干奶粉、1~5% 热灭活胎牛血清 (v/v) 或羊正常血清,以减少非特异性结合。
Other Notes
仅用于生命科学研究。不可用于诊断。
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法规信息
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