抗 HA-过氧化物酶，高亲和力

抗 HA-过氧化物酶，高亲和力是 HA-肽（克隆 3F10）的单克隆抗体，与过氧化物酶缀合。

The Anti-HA High Affinity antibody (clone 3F10) recognizes the same epitope as clone 12CA5, which was originally used to study how the immune system recognizes the influenza hemagglutinin protein, a surface glycoprotein required for infectivity of the human virus. However, the principal use of the Anti-HA antibody is the detection and purification of proteins whose encoding DNA sequences have been fused to the HA epitope sequence by recombinant techniques, that is epitope tagging. The ability to prepare such epitope-tagged proteins and locate them with the Anti-HA antibody in subsequent experiments has enabled researchers to determine:

• The size, cellular localization, and abundance of proteins produced by newly discovered genes.
• Post-translational modifications of proteins.
• The movement of proteins within cell membranes.
• The identity of proteins within functional protein complexes.
• The function of proteins that are unstable, difficult to purify, or share epitopes with a number of other proteins.

该表位由来自人流感病毒血凝素蛋白的氨基酸 98-106 组成。

• 使用抗 HA-过氧化物酶，高亲和力检测 HA 标记的重组蛋白：ELISA
• 蛋白质印迹

由于抗 HA 高亲和力是大鼠单克隆抗体，因此可以将其与鼠单克隆抗体一起用于双标记。

抗 HA-过氧化物酶，高亲和力（3F10）识别来自人流感血凝素（HA）蛋白的 9-氨基酸序列YPYDVPDYA。
无论其位置如何（N 末端，C 末端或内部），该表位也在融合蛋白中被识别。

稳定剂：蛋白质稳定剂
工作浓度：缀合物的工作浓度取决于应用和底物。
以下浓度应作为指导：

• 斑点印迹：50 mU/ml
• ELISA：25 mU/ml
• 蛋白质印迹：50 mU/ml

加入 1.0 ml 双蒸水，最终浓度为 25 U/mL。
使用前再水化 10 分钟。

仅用于生命科学研究。不可用于诊断。