assay
≥85% (HPLC)
form
solution
mol wt
(Mr = 1106.7 (DIG-11-UTP-Li4))
packaging
pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])
manufacturer/tradename
Roche
storage temp.
−20°C
General description
地高辛(DIG)-11-尿苷三磷酸(UTP)以四锂盐溶液形式提供，浓度为3.5mM(200nmol)或10mM(250nmol)。它被用作是SP6、T3和T7 RNA聚合酶的底物。它可以在体外转录反应中取代UTP，从而以35:65％的比例进行RNA的DIG标记。
Application
地高辛-11-UTP已用于标记以下应用中的RNA探针：
- 原位杂交
- 原位逆转录酶(RT)-聚合酶链式反应(PCR)
- 荧光原位杂交(FISH)
Biochem/physiol Actions
利用ATP、GTP、CTP、UTP和地高辛-11-UTP和相应的RNA聚合酶可分别对具有T7、SP6或T3启动子的线性模板DNA进行 体外 转录。随后，可以用抗地高辛-AP（碱性磷酸酶）Fab片段抗体、地高辛核酸检测试剂盒或地高辛核酸发光检测试剂盒检测标记的RNA。
Analysis Note
典型分析：85% DIG-11-UTP (HPLC，峰面积%)
使用DIG RNA标记试剂盒进行功能测试。
化学式：C43H61N4O22P3Li4
使用DIG RNA标记试剂盒进行功能测试。
化学式：C43H61N4O22P3Li4
Other Notes
仅用于生命科学研究。不可用于诊断。
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Isolation and functional characterization of two dioxygenases putatively involved in bixin biosynthesis in annatto (Bixa orellana L.)
<BIG>Carballo U, et al.</BIG>
PeerJ, 7 (2019)
Félix Legendre et al.
Journal of visualized experiments : JoVE, (71)(71), e50057-e50057 (2013-02-15)
Assessing the expression pattern of a gene, as well as the subcellular localization properties of its transcribed RNA, are key features for understanding its biological function during development. RNA in situ hybridization (RNA-ISH) is a powerful method used for visualizing
Studying gene expression in whole embryos by in situ hybridization: A peer-to-peer laboratory guide
<BIG>Kalra A and Xia D</BIG>
Arsenal (2017)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 3359247910 | 04061826742723 |
| 11209256910 | 04061837902062 |